Western Blot (WB)
(Western blot analysis of ITM2A Antibody (N-term) in K562 cell line lysates (35ug/lane). ITM2A (arrow) was detected using the purified Pab.)
Rabbit anti-Human Integral Membrane Protein 2A Polyclonal Antibody | anti-ITM2A antibody
Integral Membrane Protein 2A, NT (ITM2A, BRICD2A, E25A, Protein E25, UNQ603/PRO1189) (PE)
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
Integral Membrane Protein 2A; Polyclonal Antibody; NT (ITM2A; BRICD2A; E25A; Protein E25; UNQ603/PRO1189) (PE); anti-ITM2A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ITM2A.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Sequence Length
263
Applicable Applications for anti-ITM2A antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:50-1:100
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the N-terminal region of human ITM2A (KLH).
Conjugate
PE
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of ITM2A Antibody (N-term) in K562 cell line lysates (35ug/lane). ITM2A (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of ITM2A Antibody (N-term) in K562 cell line lysates (35ug/lane). ITM2A (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of ITM2A (arrow) using rabbit polyclonal ITM2A Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ITM2A gene (Lane 2).)
Western Blot (WB)
(Western blot analysis of ITM2A (arrow) using rabbit polyclonal ITM2A Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ITM2A gene (Lane 2).)
Flow Cytometry (FC/FACS)
(ITM2A Antibody (N-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(ITM2A Antibody (N-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-ITM2A antibody
References
1. Gudbjartsson, D.F., et.al., Nat. Genet. 40(5), 609-615 (2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29.741
NCBI Official Full Name
integral membrane protein 2A isoform 1
UniProt Protein Name
Integral membrane protein 2A
Protein Family
UniProt Gene Name
ITM2A
UniProt Synonym Gene Names
UNQ603/PRO1189
UniProt Entry Name
ITM2A_HUMAN
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Product Notes
The ITM2A itm2a (Catalog #AAA6482027) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Integral Membrane Protein 2A, NT (ITM2A, BRICD2A, E25A, Protein E25, UNQ603/PRO1189) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Integral Membrane Protein 2A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:50-1:100 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ITM2A itm2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integral Membrane Protein 2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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