Rabbit anti-Human FOXC2 Polyclonal Antibody | anti-FOXC2 antibody

FOXC2, CT (P198) (FOXC2, FKHL14, MFH1, Forkhead box protein C2, Forkhead-related protein FKHL14, Mesenchyme fork head protein 1, Transcription factor FKH-14) (Azide free) (HRP)

Cat. Num. AAA6299724

• Gene Names: FOXC2; LD; MFH1; MFH-1; FKHL14
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FOXC2; Polyclonal Antibody; CT (P198) (FOXC2; FKHL14; MFH1; Forkhead box protein C2; Forkhead-related protein FKHL14; Mesenchyme fork head protein 1; Transcription factor FKH-14) (Azide free) (HRP); Transcription factor FKH-14; anti-FOXC2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-FOXC2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FOXC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 190-218 amino acids from the Central region of human FOXC2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Flow Cytometry (FC/FACS)

(FOXC2 Antibody (Center P198) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(FOXC2 Antibody (Center P198) western blot analysis in WiDr,HL-60,ZR-75-1,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the FOXC2 antibody detected the FOXC2 protein (arrow).)

Related Product Information for anti-FOXC2 antibody

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of mesenchymal tissues.

Product Categories/Family for anti-FOXC2 antibody

Antibodies; Transcription Factors

References

Sanchez-Carpintero, R., et al. Genet. Med. 12(8):532-535(2010). Pappa, K.I., et al. Gynecol. Endocrinol. (2010). In press: Mortazavi, F., et al. Mol. Cancer Res. 8(5):762-774(2010). Fauret, A.L., et al. Lymphology 43(1):14-18(2010). Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4885237
• NCBI GeneID: 2303
• NCBI Accession #: NP_005242.1
• NCBI GenBank Nucleotide #: NM_005251.2
• UniProt Accession #: Q99958; Q14DA6; C6KMR9
• Molecular Weight: 53,719 Da
• NCBI Official Full Name: forkhead box protein C2
• NCBI Official Synonym Full Names: forkhead box C2 (MFH-1, mesenchyme forkhead 1)
• NCBI Official Symbol: FOXC2
• NCBI Official Synonym Symbols: LD; MFH1; MFH-1; FKHL14
• NCBI Protein Information: forkhead box protein C2; MFH-1,mesenchyme forkhead 1; transcription factor FKH-14; mesenchyme fork head protein 1; forkhead-related protein FKHL14; forkhead, Drosophila, homolog-like 14
• UniProt Protein Name: Forkhead box protein C2
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXC2
• UniProt Synonym Gene Names: FKHL14; MFH1; MFH-1 protein
• UniProt Entry Name: FOXC2_HUMAN

NCBI Description

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of mesenchymal tissues. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXC2: Transcriptional activator. Might be involved in the formation of special mesenchymal tissues. Defects in FOXC2 are the cause of lymphedema hereditary type 2 (LMPH2); also known as Meige lymphedema. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections, and physical impairment. Defects in FOXC2 are a cause of lymphedema-yellow nails (LYYN). LYYN is characterized by yellow, dystrophic, thick and slowly growing nails, associated with lymphedema and respiratory involvement. Lymphedema occurs more often in the lower limbs. It can appear at birth or later in life. Onset generally follows the onset of ungual abnormalities. Defects in FOXC2 are a cause of lymphedema-distichiasis (LYD). LYD is characterized by primary limb lymphedema usually starting at puberty (but in some cases later or at birth) and associated with distichiasis (double rows of eyelashes, with extra eyelashes growing from the Meibomian gland orifices).

Protein type: Cell development/differentiation; Transcription factor; Apoptosis; DNA-binding

Chromosomal Location of Human Ortholog: 16q24.1

Cellular Component: nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; chromatin DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; collagen fibril organization; neural crest cell development; heart development; positive regulation of transcription, DNA-dependent; response to hormone stimulus; cardiac muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; ureteric bud development; lymphangiogenesis; ventricular cardiac muscle morphogenesis; mesoderm development; regulation of blood vessel size; somitogenesis; Notch signaling pathway; ossification; camera-type eye development; positive regulation of cell adhesion mediated by integrin; regulation of organ growth; embryonic viscerocranium morphogenesis; regulation of transcription from RNA polymerase II promoter; patterning of blood vessels; paraxial mesodermal cell fate commitment; insulin receptor signaling pathway; artery morphogenesis; embryonic heart tube development; positive regulation of transcription from RNA polymerase II promoter; blood vessel remodeling; vascular endothelial growth factor receptor signaling pathway; metanephros development

Disease: Lymphedema-distichiasis Syndrome