Rabbit anti-Human Insulin Receptor R Polyclonal Antibody | anti-INSRR antibody

Insulin Receptor R, CT (Insulin Receptor-related Protein, Insulin Receptor-related Receptor, IR-related Receptor, INSRR, IRR) (FITC)

Cat. Num. AAA6481998

• Gene Names: INSRR; IRR
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Insulin Receptor R; Polyclonal Antibody; CT (Insulin Receptor-related Protein; Insulin Receptor-related Receptor; IR-related Receptor; INSRR; IRR) (FITC); Insulin Receptor-related Protein alpha Chain; Insulin Receptor-related Protein beta Chain; EC=2.7.10.1; anti-INSRR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human INSRR.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-INSRR antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human INSRR (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-InsRR Pab in A375 cell lysate. Lane A: preimmune, Lane B: purified antibody. InsRR (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-INSRR antibody

Antibodies; Receptors; Tyrosine Kinase

References

1. Shier, P., et al., J. Biol. Chem. 264(25):14605-14608 (1989). 2. Whitmore, T.E., et al., Cytogenet. Cell Genet. 87(1-2), 93-94 (1999). 3. Hanze, J., et al., Horm. Metab. Res. 31(2-3), 77-79 (1999). 4. Shier, P., et al., Cytogenet. Cell Genet. 54(1-2), 80-81 (1990). 5. Elmlinger, M.W., et al., Regul. Pept. 84(1-3), 37-42 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 31657140
• NCBI GeneID: 3645
• NCBI Accession #: NP_055030.1
• NCBI GenBank Nucleotide #: NM_014215.2
• UniProt Accession #: P14616; O60724; Q5VZS3
• Molecular Weight: 143,720 Da
• NCBI Official Full Name: insulin receptor-related protein
• NCBI Official Synonym Full Names: insulin receptor-related receptor
• NCBI Official Symbol: INSRR
• NCBI Official Synonym Symbols: IRR
• NCBI Protein Information: insulin receptor-related protein; IR-related receptor
• UniProt Protein Name: Insulin receptor-related protein
• Protein Family: Insulin receptor-related protein
• UniProt Gene Name: INSRR
• UniProt Synonym Gene Names: IRR; IRR
• UniProt Entry Name: INSRR_HUMAN

Uniprot Description

IRR: Receptor with tyrosine-protein kinase activity. Functions as a pH sensing receptor which is activated by increased extracellular pH. Activates an intracellular signaling pathway that involves IRS1 and AKT1/PKB. Probable tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chains carry the kinase domain. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

Protein type: Protein kinase, TK; Membrane protein, integral; Kinase, protein; Protein kinase, tyrosine (receptor); EC 2.7.10.1; TK group; InsR family

Chromosomal Location of Human Ortholog: 1q21-q23

Cellular Component: integral to plasma membrane; receptor complex

Molecular Function: insulin receptor substrate binding; phosphoinositide 3-kinase binding; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; male sex determination; actin cytoskeleton reorganization; transmembrane receptor protein tyrosine kinase signaling pathway