Rabbit anti-Human Insulin-like Growth Factor Binding Protein 3 Polyclonal Antibody | anti-IGFBP3 antibody

Insulin-like Growth Factor Binding Protein 3 (IGF Binding Protein 3, IGFBP3, IGFBP-3, IBP3, IBP-3, Binding Protein 53, BP-53) (FITC)

Cat. Num. AAA6481866

• Gene Names: Igfbp3; IGF-BP3
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Insulin-like Growth Factor Binding Protein 3; Polyclonal Antibody; Insulin-like Growth Factor Binding Protein 3 (IGF Binding Protein 3; IGFBP3; IGFBP-3; IBP3; IBP-3; Binding Protein 53; BP-53) (FITC); anti-IGFBP3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IGFBP3.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 292

• Applicable Applications for anti-IGFBP3 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to amino acid residues surrounding S183 of human IGFBP3 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis using I7661-16L3 in mouse stomach tissue lysates (15ug/lane)..)

Western Blot (WB)

(Western Blot analysis using I7661-16L3 in CHO tissue lysates (15ug/lane).)

Product Categories/Family for anti-IGFBP3 antibody

Antibodies; Growth Factors; Cytokines

References

1. Muzumdar, R.H., Diabetes 55(10), 2788-2796 (2006). 2. Novosyadlyy, R., Growth Horm. IGF Res. 15(5), 313-323 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 298231182
• NCBI GeneID: 24484
• NCBI Accession #: NP_036720
• NCBI GenBank Nucleotide #: NP_036720.2
• UniProt Accession #: P15473
• Molecular Weight: 31.68
• NCBI Official Full Name: insulin-like growth factor-binding protein 3
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 3
• NCBI Official Symbol: Igfbp3
• NCBI Official Synonym Symbols: IGF-BP3
• NCBI Protein Information: insulin-like growth factor-binding protein 3
• UniProt Protein Name: Insulin-like growth factor-binding protein 3
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: Igfbp3
• UniProt Synonym Gene Names: Igfbp-3; IBP-3; IGF-binding protein 3; IGFBP-3
• UniProt Entry Name: IBP3_RAT

NCBI Description

an insulin growth factor (Igf) binding protein; involved in modulating IGF-I action [RGD, Feb 2006]

Uniprot Description

IGFBP3: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

Protein type: Cell development/differentiation; Secreted; Secreted, signal peptide

Cellular Component: extracellular space; nuclear heterochromatin; nucleus

Molecular Function: fibronectin binding; insulin-like growth factor binding; insulin-like growth factor I binding; insulin-like growth factor II binding; protein binding; protein tyrosine phosphatase activator activity

Biological Process: aging; negative regulation of cell proliferation; negative regulation of protein amino acid phosphorylation; negative regulation of smooth muscle cell migration; negative regulation of smooth muscle cell proliferation; osteoblast differentiation; positive regulation of apoptosis; positive regulation of catalytic activity; positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of MAPKKK cascade; positive regulation of myoblast differentiation; protein amino acid phosphorylation; regulation of cell growth; regulation of growth; regulation of insulin-like growth factor receptor signaling pathway