Rabbit anti-Human Insig1 Polyclonal Antibody | anti-Insig1 antibody

Insig1, NT (Insulin-induced gene 1 protein, INSIG-1, CL6, CL-6, MGC1405) (FITC)

Cat. Num. AAA6481778

• Gene Names: INSIG1; CL6; CL-6
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Insig1; Polyclonal Antibody; NT (Insulin-induced gene 1 protein; INSIG-1; CL6; CL-6; MGC1405) (FITC); anti-Insig1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human INSIG1.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-Insig1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human INSIG1 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of INSIG1 Antibody (N-term) in Hela cell line lysates (35ug/lane). INSIG1 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(INSIG1 Antibody (N-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunohistochemistry (IHC)

(INSIG1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the INSIG1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-Insig1 antibody

Antibodies; Insulin

References

1. Smith, E.M., et.al., J. Lipid Res. 51(4), 701-708 (2010). 2. Liu, X., et.al., Atherosclerosis 208(2), 421-426 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 28882053
• NCBI GeneID: 3638
• NCBI Accession #: NP_005533.2
• NCBI GenBank Nucleotide #: NM_005542.4
• UniProt Accession #: O15503; Q53XW8; Q9BUV5; A4D2N1; A8K6L0
• Molecular Weight: 29,987 Da
• NCBI Official Full Name: insulin-induced gene 1 protein isoform 1
• NCBI Official Synonym Full Names: insulin induced gene 1
• NCBI Official Symbol: INSIG1
• NCBI Official Synonym Symbols: CL6; CL-6
• NCBI Protein Information: insulin-induced gene 1 protein; INSIG-1 membrane protein
• UniProt Protein Name: Insulin-induced gene 1 protein
• Protein Family: Insulin-induced gene 1 protein
• UniProt Gene Name: INSIG1
• UniProt Entry Name: INSI1_HUMAN

Uniprot Description

INSIG1: Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase, AMFR/gp78. May play a role in growth and differentiation of tissues involved in metabolic control. May play a regulatory role during G0/G1 transition of cell growth. Belongs to the INSIG family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum

Molecular Function: protein binding

Biological Process: cell proliferation; inner ear morphogenesis; triacylglycerol metabolic process; metabolic process; middle ear morphogenesis; palate development; negative regulation of fat cell differentiation; SREBP-mediated signaling pathway; cholesterol biosynthetic process; negative regulation of fatty acid biosynthetic process