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Western Blot (WB) (Western Blot (WB) analysis of INPP5J polyclonal antibody)

Rabbit INPP5J Polyclonal Antibody | anti-INPP5J antibody

INPP5J Polyclonal Antibody

Gene Names
INPP5J; PIPP; INPP5; PIB5PA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
INPP5J; Polyclonal Antibody; INPP5J Polyclonal Antibody; Phosphatidylinositol 4; 5-bisphosphate 5-phosphatase A; Inositol polyphosphate 5-phosphatase J; PIB5PA; PIPP; anti-INPP5J antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
INPP5J polyclonal antibody detects endogenous levels of INPP5J protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
638
Applicable Applications for anti-INPP5J antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:100
IF: 1:10-1:100
Immunogen
Recombinant full length Human INPP5J.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of INPP5J polyclonal antibody)

Western Blot (WB) (Western Blot (WB) analysis of INPP5J polyclonal antibody)
Related Product Information for anti-INPP5J antibody
Inositol and phosphatidylinositol phosphates are important for numerous cellular processes, including neuronal survival and signal transductions from growth factors, neurotransmitters and G protein coupled receptors. INPP5J (inositol polyphosphate-5-phosphatase J), also known as phosphatidylinositol 4, 5-bisphosphate 5-phosphatase A, PIPP (proline-rich inositol polyphosphate 5-phosphatase), PIB5PA or INPP5, is a 1, 006 amino acid cytoplasmic protein that assists in the conversion of inositol 1, 4-bisphosphate from inositol 1, 4, 5-trisphosphate. Expressed in lung, stomach, kidney, brain, small intestine and heart, INPP5J localizes to membrane ruffles, where it may also participate in modulating inositol and phosphatidylinositol polyphosphate-binding proteins. Encoded by a gene located on human chromosome 22, INPP5J undergoes alternative splicing to produce three isoforms.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 107kDa
NCBI Official Full Name
phosphatidylinositol 4,5-bisphosphate 5-phosphatase A isoform c
NCBI Official Synonym Full Names
inositol polyphosphate-5-phosphatase J
NCBI Official Symbol
INPP5J
NCBI Official Synonym Symbols
PIPP; INPP5; PIB5PA
NCBI Protein Information
phosphatidylinositol 4,5-bisphosphate 5-phosphatase A
UniProt Protein Name
Phosphatidylinositol 4,5-bisphosphate 5-phosphatase A
UniProt Gene Name
INPP5J
UniProt Synonym Gene Names
PIB5PA; PIPP
UniProt Entry Name
PI5PA_HUMAN

Uniprot Description

PI5PA: Inositol 5-phosphatase, which converts inositol 1,4,5- trisphosphate to inositol 1,4-bisphosphate. Also converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4- phosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate in vitro. May be involved in modulation of the function of inositol and phosphatidylinositol polyphosphate- binding proteins that are present at membranes ruffles. Belongs to the inositol 1,4,5-trisphosphate 5- phosphatase type II family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.56; Phosphatase (non-protein); Carbohydrate Metabolism - inositol phosphate

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: ruffle; growth cone; cytoplasm; plasma membrane; dendritic shaft; cytosol

Molecular Function: inositol-polyphosphate 5-phosphatase activity; SH3 domain binding

Biological Process: inositol phosphate metabolic process; phospholipid metabolic process; negative regulation of microtubule polymerization; phosphatidylinositol biosynthetic process; negative regulation of peptidyl-serine phosphorylation; phosphoinositide dephosphorylation

Research Articles on INPP5J

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Product Notes

The INPP5J inpp5j (Catalog #AAA3011635) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The INPP5J Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's INPP5J can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:100 IF: 1:10-1:100. Researchers should empirically determine the suitability of the INPP5J inpp5j for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "INPP5J, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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