Rabbit anti-Human, Mouse Inositol 1,4,5-trisphosphate 3-kinase A Polyclonal Antibody | anti-IP3KA antibody

Inositol 1,4,5-trisphosphate 3-kinase A, CT (Inositol-trisphosphate 3-kinase A, InsP 3-kinase A, IP3 3-kinase A, IP3K A, IP3KA, IP3-3KA, ITPKA) (FITC)

Cat. Num. AAA6481756

• Gene Names: ITPKA; IP3KA; IP3-3KA
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Inositol 1; 4; 5-trisphosphate 3-kinase A; Polyclonal Antibody; CT (Inositol-trisphosphate 3-kinase A; InsP 3-kinase A; IP3 3-kinase A; IP3K A; IP3KA; IP3-3KA; ITPKA) (FITC); EC=2.7.1.127; anti-IP3KA antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ITPKA. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-IP3KA antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human ITPKA (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of hITPKA-E360 in K562 cell line and mouse lung tissue lysates (35ug/lane). ITPKA (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-IP3KA antibody

Antibodies; Protein Kinases

References

1. Gonzalez, B., et al., Mol. Cell 15(5):689-701 (2004). 2. Mishra, J., et al., Biophys. J. 83(3):1298-1316 (2002). 3. Schell, M.J., et al., J. Biol. Chem. 276(40):37537-37546 (2001). 4. Communi, D., et al., EMBO J. 16(8):1943-1952 (1997). 5. Woodring, P.J., et al., J. Biol. Chem. 272(48):30447-30454 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 4504789
• NCBI GeneID: 3706
• NCBI Accession #: NP_002211
• NCBI GenBank Nucleotide #: NM_002220.2
• UniProt Accession #: P23677; Q8TAN3
• Molecular Weight: 51,009 Da
• NCBI Official Full Name: inositol-trisphosphate 3-kinase A
• NCBI Official Synonym Full Names: inositol-trisphosphate 3-kinase A
• NCBI Official Symbol: ITPKA
• NCBI Official Synonym Symbols: IP3KA; IP3-3KA
• NCBI Protein Information: inositol-trisphosphate 3-kinase A
• UniProt Protein Name: Inositol-trisphosphate 3-kinase A
• UniProt Gene Name: ITPKA
• UniProt Synonym Gene Names: IP3 3-kinase A; IP3K A
• UniProt Entry Name: IP3KA_HUMAN

NCBI Description

Regulates inositol phosphate metabolism by phosphorylation of second messenger inositol 1,4,5-trisphosphate to Ins(1,3,4,5)P4. The activity of the inositol 1,4,5-trisphosphate 3-kinase is responsible for regulating the levels of a large number of inositol polyphosphates that are important in cellular signaling. Both calcium/calmodulin and protein phosphorylation mechanisms control its activity. It is also a substrate for the cyclic AMP-dependent protein kinase, calcium/calmodulin- dependent protein kinase II, and protein kinase C in vitro.[provided by RefSeq, Apr 2011]

Uniprot Description

ITPKA: Regulates inositol phosphate metabolism by phosphorylation of second messenger inositol 1,4,5-trisphosphate to Ins(1,3,4,5)P4. The activity of the inositol 1,4,5-trisphosphate 3-kinase is responsible for regulating the levels of a large number of inositol polyphosphates that are important in cellular signaling. Both calcium/calmodulin and protein phosphorylation mechanisms control its activity. It is also a substrate for the cyclic AMP-dependent protein kinase, calcium/calmodulin- dependent protein kinase II, and protein kinase C in vitro.[provided by RefSeq, Apr 2011]

Protein type: Kinase, lipid; Motility/polarity/chemotaxis; Carbohydrate Metabolism - inositol phosphate; EC 2.7.1.127

Chromosomal Location of Human Ortholog: 15q15.1

Cellular Component: dendritic spine; cytosol

Molecular Function: calmodulin binding; calmodulin-dependent protein kinase activity; Rac GTPase binding; inositol trisphosphate 3-kinase activity; ATP binding

Biological Process: inositol phosphate metabolic process; inositol metabolic process; regulation of synaptic plasticity; signal transduction; actin cytoskeleton organization and biogenesis; protein amino acid phosphorylation