Rabbit Inhibitor of nuclear factor kappa-B kinase subunit beta Polyclonal Antibody | anti-IKBKB antibody

Anti-IKK beta Antibody

Cat. Num. AAA177446

• Gene Names: IKBKB; IKK2; IKKB; IMD15; NFKBIKB; IKK-beta
• Reactivity: Human. No cross reactivity with other proteins.
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: Inhibitor of nuclear factor kappa-B kinase subunit beta; Polyclonal Antibody; Anti-IKK beta Antibody; I kappa B kinase 2 antibody; I kappa B kinase beta antibody; I-kappa-B kinase 2 antibody; I-kappa-B-kinase beta antibody; IkBKB antibody; IKK 2 antibody; IKK B antibody; IKK beta antibody; IKK-B antibody; IKK-beta antibody; IKK2 antibody; IKKB antibody; IKKB_HUMAN antibody; IMD15 antibody; Inhibitor of kappa light chain gene enhancer in B cells antibody; Inhibitor of kappa light polypeptide gene enhancer in B cells antibody; Inhibitor of kappa light polypeptide gene enhancer in B cells kinase beta antibody; Inhibitor of kappa light polypeptide gene enhancer in B cells; kinase beta antibody; Inhibitor of nuclear factor kappa B kinase beta subunit antibody; Inhibitor of nuclear factor kappa B kinase subunit beta antibody; Inhibitor of nuclear factor kappa-B kinase subunit beta antibody; MGC131801 antibody; NFKBIKB antibody; Nuclear factor NF kappa B inhibitor kinase beta antibody; Nuclear factor NF-kappa-B inhibitor kinase beta antibody; Nuclear factor of kappa light chain gene enhancer in B cells inhibitor antibody; inhibitor of kappa light polypeptide gene enhancer in B-cells; kinase beta; anti-IKBKB antibody

• Host: Rabbit
• Reactivity: Human. No cross reactivity with other proteins.
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 754

• Applicable Applications for anti-IKBKB antibody: Western Blot (WB), Immunohistochemistry (IHC-P)
• Application Notes: Western Blot: Concentration: 0.1-0.5mug/ml; Tested Species: Human; Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1mug/ml; Tested Species: Human
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Immunogen: E.coli-derived human IKK beta recombinant protein (Position: E398-S756). Human IKK beta shares 91% and 90% amino acid (aa) sequences identity with mouse and rat IKK beta, respectively.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- IKK beta antibody, MBS177446, Western blottingAll lanes: Anti IKK beta (MBS177446) at 0.5ug/mlLane 1: HEPG2 Whole Cell Lysate at 40ugLane 2: COLO320 Whole Cell Lysate at 40ugLane 3: M231 Whole Cell Lysate at 40ugLane 4: HT1080 Whole Cell Lysate at 40ugPredicted bind size: 87KDObserved bind size: 87KD )

Immunohistochemistry (IHC)

(Anti- IKK beta antibody, MBS177446,IHC(P) IHC(P): Human Lung Cancer Tissue )

Western Blot (WB)

(Anti- IKK beta antibody, MBS177446, Western blottingAll lanes: Anti IKK beta (MBS177446) at 0.5ug/mlWB: Recombinant Human IKK beta Protein 0.5ngPredicted bind size: 43KDObserved bind size: 43KD )

Related Product Information for anti-IKBKB antibody

Description: Rabbit IgG polyclonal antibody for Inhibitor of nuclear factor kappa-B kinase subunit beta(IKBKB) detection. Tested with WB, IHC-P in Human.
Background: IKBKB(Inhibitor of Kappa Light Chain Gene Enhancer in B Cells, Kinase of, Beta), also known as IKKB or NFKBIKB, is a protein that in humans is encoded by the IKBKB gene. This gene is mapped to chromosome 8p12-p11 by FISH. It is found that mutations in IKK2 had a more pronounced effect upon NFKB activation than did comparable mutations in IKK1. The activity of various antiinflammatory agents on the IKK complex has been texted, it has been found that aspirin and sodium salicylate specifically inhibit IKK-beta activity in vitro and in vivo by binding to IKK-beta to reduce ATP binding.

NCBI and Uniprot Product Information

• NCBI GI #: 299758411
• NCBI GeneID: 3551
• NCBI Accession #: NP_001177649.1
• NCBI GenBank Nucleotide #: NM_001190720.2
• UniProt Accession #: O14920; O75327; B4DZ30; B4E0U4
• Molecular Weight: 79,509 Da
• NCBI Official Full Name: inhibitor of nuclear factor kappa-B kinase subunit beta isoform 2
• NCBI Official Synonym Full Names: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
• NCBI Official Symbol: IKBKB
• NCBI Official Synonym Symbols: IKK2; IKKB; IMD15; NFKBIKB; IKK-beta
• NCBI Protein Information: inhibitor of nuclear factor kappa-B kinase subunit beta; IKK-B; I-kappa-B kinase 2; I-kappa-B-kinase beta; nuclear factor NF-kappa-B inhibitor kinase beta
• UniProt Protein Name: Inhibitor of nuclear factor kappa-B kinase subunit beta
• Protein Family: Inhibitor of nuclear factor kappa-B kinase
• UniProt Gene Name: IKBKB
• UniProt Synonym Gene Names: IKKB; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; IKK2; NFKBIKB
• UniProt Entry Name: IKKB_HUMAN

NCBI Description

The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

IKKB: a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Preferentially found as a heterodimer with IKK-alpha but also as an homodimer.

Protein type: EC 2.7.11.10; Kinase, protein; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; IKK family

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: internal side of plasma membrane; cytoplasm; IkappaB kinase complex; cytosol; nucleus; lipid raft

Molecular Function: protein serine/threonine kinase activity; protein binding; protein homodimerization activity; IkappaB kinase activity; protein heterodimerization activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; T cell receptor signaling pathway; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 5 signaling pathway; B cell homeostasis; positive regulation of interferon type I production; inflammatory response; toll-like receptor 4 signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; response to virus; MyD88-independent toll-like receptor signaling pathway; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; serine phosphorylation of STAT protein; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; I-kappaB phosphorylation; negative regulation of apoptosis

Disease: Immunodeficiency 15