Human Inhibitor of nuclear factor kappa-B kinase subunit beta ELISA Kit | IKBKB elisa kit

Human Inhibitor of nuclear factor kappa-B kinase subunit beta ELISA Kit

Cat. Num. AAA760304

• Gene Names: IKBKB; IKK2; IKKB; IMD15; NFKBIKB; IKK-beta
• Reactivity: Human
• Synonyms: Inhibitor of nuclear factor kappa-B kinase subunit beta; Human Inhibitor of nuclear factor kappa-B kinase subunit beta ELISA Kit; IKBKB/Inhibitor of nuclear factor kappa-B kinase subunit beta/I-kappa-B-kinase beta/IKK-B/IKK-beta/IkBKB/I-kappa-B kinase 2/IKK2/Nuclear factor NF-kappa-B inhibitor kinase beta/NFKBIKB/IKKB; IKBKB elisa kit

• Reactivity: Human
• Sequence Length: 754

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 1.56-100ng/ml
• Sensitivity: 0.938ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for IKBKB elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human IKBKB antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human IKBKB antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IKBKB amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human IKBKB can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 299758411
• NCBI GeneID: 3551
• NCBI Accession #: NP_001177649.1
• NCBI GenBank Nucleotide #: NM_001190720.2
• Molecular Weight: 79,509 Da
• NCBI Official Full Name: inhibitor of nuclear factor kappa-B kinase subunit beta isoform 2
• NCBI Official Synonym Full Names: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
• NCBI Official Symbol: IKBKB
• NCBI Official Synonym Symbols: IKK2; IKKB; IMD15; NFKBIKB; IKK-beta
• NCBI Protein Information: inhibitor of nuclear factor kappa-B kinase subunit beta
• UniProt Protein Name: Inhibitor of nuclear factor kappa-B kinase subunit beta
• Protein Family: Inhibitor of nuclear factor kappa-B kinase
• UniProt Gene Name: IKBKB
• UniProt Synonym Gene Names: IKKB; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; IKK2; NFKBIKB
• UniProt Entry Name: IKKB_HUMAN

NCBI Description

The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

IKKB: a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Preferentially found as a heterodimer with IKK-alpha but also as an homodimer.

Protein type: EC 2.7.11.10; Protein kinase, Other; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Other group; IKK family

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: cytoplasm; cytosol; IkappaB kinase complex; internal side of plasma membrane; lipid raft; nucleus

Molecular Function: ATP binding; IkappaB kinase activity; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity

Biological Process: activation of NF-kappaB transcription factor; B cell homeostasis; cell surface receptor linked signal transduction; cortical actin cytoskeleton organization and biogenesis; I-kappaB kinase/NF-kappaB cascade; I-kappaB phosphorylation; inflammatory response; innate immune response; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of apoptosis; nerve growth factor receptor signaling pathway; neurite development; positive regulation of cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; regulation of phosphorylation; response to drug; response to lipopolysaccharide; response to toxin; response to virus; serine phosphorylation of STAT protein; skeletal muscle contraction; stimulatory C-type lectin receptor signaling pathway; stress-activated MAPK cascade; T cell receptor signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; tumor necrosis factor-mediated signaling pathway

Disease: Immunodeficiency 15