Western Blot (WB)
(Staining (2ug/ml) of HepG2 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.)
Goat ING2/ING1L Polyclonal Antibody | anti-ING2 antibody
Goat anti-ING2/ING1L Antibody
Western Blot: Approx 30kDa band observed in hepatocarcinoma HEPG2 lysates (calculated MW of 32.8kDa according to NP_001555). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
(Staining (2ug/ml) of HepG2 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (2ug/ml) of HepG2 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the inhibitor of growth (ING) family. Members of the ING family associate with and modulate the activity of histone acetyltransferase (HAT) and histone deacetylase (HDAC) complexes and function in DNA repair and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
Uniprot Description
ING2: Seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. Component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. ING2 activity seems to be modulated by binding to phosphoinositides (PtdInsPs). Belongs to the ING family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 4q35.1
Cellular Component: CCAAT-binding factor complex; cytoplasm; Golgi apparatus; nucleoplasm; nucleus; plasma membrane; Sin3 complex
Molecular Function: chromatin binding; DNA binding; methylated histone residue binding; phosphoinositide binding; protein binding; protein complex binding; zinc ion binding
Biological Process: chromatin modification; male germ-line stem cell division; male meiosis I; negative regulation of cell proliferation; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor beta receptor signaling pathway; regulation of growth; regulation of transcription, DNA-dependent; signal transduction; sperm motility; spermatid development; spermatogenesis; transcription, DNA-dependent
Research Articles on ING2
Similar Products
Product Notes
The ING2 ing2 (Catalog #AAA420533) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ING2/ING1L Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's ING2/ING1L can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 1000. Western Blot: Approx 30kDa band observed in hepatocarcinoma HEPG2 lysates (calculated MW of 32.8kDa according to NP_001555). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the ING2 ing2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DKSTEKTKKD RRSR. It is sometimes possible for the material contained within the vial of "ING2/ING1L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
