Rabbit anti-Human, Mouse ING1, p33 Polyclonal Antibody | anti-ING1 antibody

ING1, p33 (Inhibitor of Growth 1)

Cat. Num. AAA620169

• Gene Names: ING1; p33; p47; p33ING1; p24ING1c; p33ING1b; p47ING1a
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: Serum; Serum
• Synonyms: ING1; p33; Polyclonal Antibody; p33 (Inhibitor of Growth 1); Anti -ING1; anti-ING1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects a ~33kD protein, corresponding to the apparent molecular mass of p33 ING1 on SDS-PAGE immunoblots. Species Crossreactivity: Human and Mouse. Not crossreactive with any other ING family.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid in TBS, 0.1% sodium azide.

• Applicable Applications for anti-ING1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, ELISA and Immunofluorescence.; Dilution: Western Blot: 0.25mg/ml was sufficient for detection in lysates prepared from human melanoma cell lines by Western Blot analysis.
• Immunogen: Mouse p33 ING1 N-terminal peptide -KLH conjugates
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-ING1 antibody

The p33ING1 protein encodes a 33-kD, 294-amino acid protein that displays the characteristics of a tumor suppressor gene. The p33ING1 protein is a regulator of cell cycle, senescence, and apoptosis. It can mediate growth arrest, anchor dependent growth and influence chemosensitivity (1-4). It also has a proposed role as a growth regulator, which is consistent with its location in the nucleus (5). It has been demonstrated that the ING1 gene has three exons and that four mRNA variants are transcribed from three different promoter regions (p33 ING1, p24 ING1, p27 ING1, p47 IG1) (6, 7). ING1 can possibly influence the above biological functions depending on which variant is expressed (1-4).

Product Categories/Family for anti-ING1 antibody

Antibodies; Abs to Tumor Suppressors

NCBI and Uniprot Product Information

• NCBI GI #: 38201667
• NCBI GeneID: 3621
• NCBI Accession #: NP_937862.1
• NCBI GenBank Nucleotide #: NM_198219.1
• UniProt Accession #: Q5T9H0; Q9UK53
• Molecular Weight: 31,864 Da
• NCBI Official Full Name: inhibitor of growth protein 1 isoform A
• NCBI Official Synonym Full Names: inhibitor of growth family, member 1
• NCBI Official Symbol: ING1
• NCBI Official Synonym Symbols: p33; p47; p33ING1; p24ING1c; p33ING1b; p47ING1a
• NCBI Protein Information: inhibitor of growth protein 1; OTTHUMP00000018703; OTTHUMP00000018704; OTTHUMP00000018705; OTTHUMP00000018706; growth inhibitor ING1; tumor suppressor ING1; growth inhibitory protein ING1
• UniProt Protein Name: Inhibitor of growth family, member 1
• Protein Family: Inhibitor of growth protein
• UniProt Gene Name: ING1
• UniProt Entry Name: Q5T9H0_HUMAN

NCBI Description

This gene encodes a tumor suppressor protein that can induce cell growth arrest and apoptosis. The encoded protein is a nuclear protein that physically interacts with the tumor suppressor protein TP53 and is a component of the p53 signaling pathway. Reduced expression and rearrangement of this gene have been detected in various cancers. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Uniprot Description

ING1: Cooperates with p53/TP53 in the negative regulatory pathway of cell growth by modulating p53-dependent transcriptional activation. Implicated as a tumor suppressor gene. Defects in ING1 are a cause of head and neck squamous cell carcinomas (HNSCC); also known as squamous cell carcinoma of the head and neck. Belongs to the ING family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: nucleus

Molecular Function: protein binding; zinc ion binding; methylated histone residue binding

Biological Process: negative regulation of cell proliferation; protein import into nucleus; positive regulation of transcription, DNA-dependent; negative regulation of cell growth; chromatin modification; cell cycle

Disease: Squamous Cell Carcinoma, Head And Neck