Rabbit Influenza A Polyclonal Antibody | anti-HA antibody

Influenza A (Swine H1N1) Hemagglutinin (A/California/14/2009) (FITC)

Cat. Num. AAA6481635

• Applications: ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Influenza A; Polyclonal Antibody; Influenza A (Swine H1N1) Hemagglutinin (A/California/14/2009) (FITC); anti-HA antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the hemagglutinin peptide from the swine-origin Influenza A/California/14/2009. Does not cross-react with the corresponding peptide from seasonal Influenza A/Brisbane/59/2007 (H1N1).
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-HA antibody: ELISA (EIA)
• Application Notes: ELISA: 1ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to the hemagglutinin protein of the swine-origin Influenza A/California/14/2009 (H1N1) (ACQ76314)
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

ELISA (EIA)

(ELISA results at 1ug/ml and the blocking and corresponding peptides at 50, 10, 2 and 0ng.)

Product Categories/Family for anti-HA antibody

Antibodies; Infectious Disease Influenza

References

1. Thompson, W.W., et al., (2003), "Mortality associated with influenza and respiratory syncytial virus in the United States, " JAMA, 289:179-186. 2. Dawood, F.S., et al., (2009), "Novel Swine-Origin Influenza A (H1N1) Virus Investigation Team, Emergence of a novel swine-origin influenza A (H1N1) virus in humans, " N. Engl. J. Med., 360:2605-2615. 3. Butler D., (2009), "Swine flu goes global, " Nature, 458:1082-1083. 4. Morens, D.M., et al., (2009), "The Persistent Legacy of the 1918 Influenza Virus, " N. Engl. J. Med., June 29. 5. Garten R.J., et al., (2009), "Antigenic and Genetic Characteristics of Swine-Origin 2009 A (H1N1) Influenza Viruses Circulating in Humans, " Science, May 22.

NCBI and Uniprot Product Information

• NCBI GI #: 229535834
• UniProt Accession #: C4AL34
• Molecular Weight: 63,236 Da
• NCBI Official Full Name: hemagglutinin
• UniProt Protein Name: Hemagglutinin
• Protein Family: Protein
• UniProt Gene Name: HA
• UniProt Entry Name: C4AL34_9INFA

Uniprot Description

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.