Typical Testing Data/Standard Curve (for reference only)
Rat Glucose Transporter 2 (GLUT2) ELISA Kit | GLUT2 elisa kit
Rat Glucose Transporter 2 (GLUT2) ELISA Kit
Gene Names
Slc2a2; GTT2; Glut2
Reactivity
Rat
Synonyms
Glucose Transporter 2 (GLUT2); Rat Glucose Transporter 2 (GLUT2) ELISA Kit; SLC2A2; SLC2-A2; Solute carrier family 2; facilitated glucose transporter member 2; Glucose transporter type 2; liver; GLUT2 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of GLUT2. No significant cross-reactivity or interference between GLUT2 and analogues was observed.
Sequence Length
522
Samples
Tissue homogenates, cell lysates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.78-50ng/mL
Sensitivity
<0.33ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GLUT2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GLUT2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GLUT2 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of GLUT2 in rat tissue homogenates, cell lysates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GLUT2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GLUT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GLUT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GLUT2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GLUT2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GLUT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GLUT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GLUT2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
57,086 Da
NCBI Official Full Name
Solute carrier family 2, facilitated glucose transporter member 2
NCBI Official Synonym Full Names
solute carrier family 2 member 2
NCBI Official Symbol
Slc2a2
NCBI Official Synonym Symbols
GTT2; Glut2
NCBI Protein Information
solute carrier family 2, facilitated glucose transporter member 2
UniProt Protein Name
Solute carrier family 2, facilitated glucose transporter member 2
UniProt Gene Name
Slc2a2
UniProt Synonym Gene Names
Glut-2; Glut2; GLUT-2
NCBI Description
acts as a sodium-independent glucose transporter; plays a role in intestinal glucose transport [RGD, Feb 2006]
Uniprot Description
Facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na+/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney.
Research Articles on GLUT2
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Product Notes
The Rat GLUT2 slc2a2 (Catalog #AAA457035) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA457035 ELISA Kit recognizes Rat GLUT2. It is sometimes possible for the material contained within the vial of "Glucose Transporter 2 (GLUT2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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