Rabbit anti-Human, Mouse IMP3 Polyclonal Antibody | anti-IMP3 antibody

IMP3, CT (IMP3, C15orf12, MRPS4, U3 small nucleolar ribonucleoprotein protein IMP3, BRMS2) (PE)

Cat. Num. AAA6310409

• Gene Names: IMP3; BRMS2; MRPS4; C15orf12
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IMP3; Polyclonal Antibody; CT (IMP3; C15orf12; MRPS4; U3 small nucleolar ribonucleoprotein protein IMP3; BRMS2) (PE); BRMS2; anti-IMP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-IMP3 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: IMP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155~184 amino acids from the C-terminal region of human IMP3.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of IMP3 Antibody (C-term) in Hela cell line lysates (35ug/lane). IMP3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(IMP3 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IMP3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(IMP3 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-IMP3 antibody

This gene encodes the human homolog of the yeast Imp3 protein. The protein localizes to the nucleoli and interacts with the U3 snoRNP complex. The protein contains an S4 domain.

Product Categories/Family for anti-IMP3 antibody

Antibodies; Nuclear Proteins

References

Li, D., et al. Ann. Surg. Oncol. 16(12):3499-3506(2009). Walter, O., et al. Hum. Pathol. 40(11):1528-1533(2009). Riener, M.O., et al. Hum. Pathol. 40(10):1377-1383(2009). Noske, A., et al. Int. J. Gynecol. Pathol. 28(3):203-210(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 8922794
• NCBI GeneID: 55272
• NCBI Accession #: NP_060755.1
• NCBI GenBank Nucleotide #: NM_018285.3
• UniProt Accession #: Q9NV31; Q53G10; B3KQ67
• Molecular Weight: 21,850 Da
• NCBI Official Full Name: U3 small nucleolar ribonucleoprotein protein IMP3
• NCBI Official Synonym Full Names: IMP3, U3 small nucleolar ribonucleoprotein
• NCBI Official Symbol: IMP3
• NCBI Official Synonym Symbols: BRMS2; MRPS4; C15orf12
• NCBI Protein Information: U3 small nucleolar ribonucleoprotein protein IMP3; IMP3, U3 small nucleolar ribonucleoprotein, homolog; U3 snoRNP protein 3 homolog; U3 snoRNP protein IMP3
• UniProt Protein Name: U3 small nucleolar ribonucleoprotein protein IMP3
• Protein Family: U3 small nucleolar ribonucleoprotein protein
• UniProt Gene Name: IMP3
• UniProt Synonym Gene Names: C15orf12; MRPS4; U3 snoRNP protein IMP3
• UniProt Entry Name: IMP3_HUMAN

NCBI Description

This gene encodes the human homolog of the yeast Imp3 protein. The protein localizes to the nucleoli and interacts with the U3 snoRNP complex. The protein contains an S4 domain. [provided by RefSeq, Jul 2008]

Uniprot Description

IMP3: Component of the 60-80S U3 small nucleolar ribonucleoprotein (U3 snoRNP). Required for the early cleavages during pre-18S ribosomal RNA processing. Belongs to the ribosomal protein S4P family.

Protein type: RNA processing; RNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: 15q24

Cellular Component: small subunit processome; small ribosomal subunit; nucleolus; nucleus

Molecular Function: snoRNA binding; rRNA binding; protein binding; structural constituent of ribosome

Biological Process: positive regulation of translational fidelity; rRNA processing