Goat IMP1/ZBP1 Polyclonal Antibody | anti-IGF2BP1 antibody

Goat anti-IMP1/ZBP1 Antibody

Cat. Num. AAA422379

• Gene Names: IGF2BP1; IMP1; ZBP1; CRDBP; IMP-1; CRD-BP; VICKZ1
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: IMP1/ZBP1; Polyclonal Antibody; Goat anti-IMP1/ZBP1 Antibody; IGF2BP1; insulin-like growth factor 2 mRNA binding protein 1; CRD-BP; CRDBP; IMP-1; IMP1; VICKZ1; ZBP1; IGF II mRNA binding protein 1; IGF2 mRNA-binding protein 1; VICKZ family member 1; coding region determinant-binding protein; zipcode-binding protein 1; coding region determinant-binding protein antibody; CRDBP antibody; CRD-BP antibody; IGF II mRNA binding protein 1 antibody; IGF2 mRNA-binding protein 1 antibody; IMP1 antibody; IMP-1 antibody; insulin-like growth factor 2 mRNA binding protein 1 antibody; VICKZ family member 1 antibody; VICKZ1 antibody; ZBP1 antibody; zipcode-binding protein 1 antibody; IGF2BP1 antibody; IMP1 / ZBP1; anti-IGF2BP1 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: EKVFAEHKISYSGQ
• Sequence Length: 577

• Applicable Applications for anti-IGF2BP1 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 16000.; Western Blot: Approx 65kDa band observed in lysates of cell line K562 (calculated MW of 63.5 kDa according to NP_006537.3). Recommended concentration: 0.3-1 μg/ml. Primary incubation was 1 hour.; IHC: Paraffin embedded Human Breast. Recommended concentration: 3.75 μg/ml.
• Immunogen: Peptide with sequence C-EKVFAEHKISYSGQ, from the internal region of the protein sequence according to NP_006537.3; NP_001153895.1.
• Epitope: Internal region
• Note: This antibody is expected to recognize both reported isoforms (NP_006537.3; NP_001153895.1).
• Preparation and Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Western Blot (WB)

(MBS422379 (0.3 μg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.)

Immunohistochemistry (IHC)

(MBS422379 (3.75 μg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 56237027
• NCBI GeneID: 10642
• NCBI Accession #: NP_006537.3; NP_001153895.1
• NCBI GenBank Nucleotide #: NM_006546.3
• NCBI Official Full Name: insulin-like growth factor 2 mRNA-binding protein 1 isoform 1
• NCBI Official Synonym Full Names: insulin like growth factor 2 mRNA binding protein 1
• NCBI Official Symbol: IGF2BP1
• NCBI Official Synonym Symbols: IMP1; ZBP1; CRDBP; IMP-1; CRD-BP; VICKZ1
• NCBI Protein Information: insulin-like growth factor 2 mRNA-binding protein 1
• UniProt Protein Name: Insulin-like growth factor 2 mRNA-binding protein 1
• Protein Family: Insulin-like growth factor 2 mRNA-binding protein
• UniProt Gene Name: IGF2BP1
• UniProt Synonym Gene Names: CRDBP; VICKZ1; ZBP1; IGF2 mRNA-binding protein 1; IMP-1; IMP1; CRD-BP; ZBP-1
• UniProt Entry Name: IF2B1_HUMAN

NCBI Description

This gene encodes a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

IGF2BP1: a conserved trans-acting factor that can control translation of specific mRNAs in vivo. Contains four K homology (KH) domains and two RNA recognition motifs. It functions by binding to the 5' UTR of the target mRNAs and repressing translation. Target mRNAs include those encoding beta-actin and insulin-like growth factor 2 (IGF2). Tyrosine phosphorylation apparently abolishes translational repression of actin expression by decreasing its binding beta-actin mRNA. May have a role in progression of ovarian cancer. Contains nuclear export signals within the RNA-binding KH2 and KH4 domains. Aberrant expression may interfere with c-myc regulation.

Protein type: Translation; RNA-binding

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: axon; cytoplasm; cytosol; dendritic spine; filopodium; growth cone; lamellipodium; nucleus; perinuclear region of cytoplasm; ribonucleoprotein complex; stress granule

Molecular Function: mRNA 3'-UTR binding; mRNA 5'-UTR binding; mRNA binding; nucleotide binding; protein binding; translation regulator activity

Biological Process: gene expression; mRNA transport; negative regulation of translation; pallium cell proliferation in the forebrain; regulation of cytokine biosynthetic process