Human Immune Receptor Expressed On Myeloid Cells 1 (IREM1) ELISA Kit | IREM1 elisa kit

Human Immune Receptor Expressed On Myeloid Cells 1 (IREM1) ELISA Kit

Cat. Num. AAA2703254

• Gene Names: CD300LF; CLM1; NKIR; CLM-1; IREM1; LMIR3; CD300f; IREM-1; IgSF13
• Reactivity: Human
• Synonyms: Immune Receptor Expressed On Myeloid Cells 1 (IREM1); Human Immune Receptor Expressed On Myeloid Cells 1 (IREM1) ELISA Kit; CD300f; CD300LF; NKIR; IGSF13; CLM1; CD300 Antigen Like Family Member F; CMRF35-Like Molecule 1; Immunoglobulin superfamily member 13; NK inhibitory receptor; IREM1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Immune Receptor Expressed On Myeloid Cells 1 (IREM1).; No significant cross-reactivity or interference between Immune Receptor Expressed On Myeloid Cells 1 (IREM1) and analogues was observed.
• Sequence Length: 293

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immune Receptor Expressed On Myeloid Cells 1 (IREM1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immune Receptor Expressed On Myeloid Cells 1 (IREM1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IREM1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immune Receptor Expressed On Myeloid Cells 1 (IREM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immune Receptor Expressed On Myeloid Cells 1 (IREM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immune Receptor Expressed On Myeloid Cells 1 (IREM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immune Receptor Expressed On Myeloid Cells 1 (IREM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 30962591
• NCBI GeneID: 146722
• Molecular Weight: 32,628 Da
• NCBI Official Full Name: immune receptor expressed on myeloid cells splice variant 1
• NCBI Official Synonym Full Names: CD300 molecule like family member f
• NCBI Official Symbol: CD300LF
• NCBI Official Synonym Symbols: CLM1; NKIR; CLM-1; IREM1; LMIR3; CD300f; IREM-1; IgSF13
• NCBI Protein Information: CMRF35-like molecule 1
• UniProt Protein Name: CMRF35-like molecule 1
• UniProt Gene Name: CD300LF
• UniProt Synonym Gene Names: CD300F; CLM1; IGSF13; IREM1; NKIR; CLM-1; IREM-1; IgSF13

NCBI Description

This gene encodes a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. The encoded protein is an inhibitory receptor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

Acts as an inhibitory receptor for myeloid cells and mast cells (PubMed:15549731). Positively regulates the phagocytosis of apoptotic cells (efferocytosis) via phosphatidylserine (PS) recognition; recognizes and binds PS as a ligand which is expressed on the surface of apoptotic cells. Plays an important role in the maintenance of immune homeostasis, by promoting macrophage-mediated efferocytosis and by inhibiting dendritic cell-mediated efferocytosis (). Negatively regulates Fc epsilon receptor-dependent mast cell activation and allergic responses via binding to ceramide and sphingomyelin which act as ligands (PubMed:24035150). May act as a coreceptor for interleukin 4 (IL-4). Associates with and regulates IL-4 receptor alpha-mediated responses by augmenting IL-4- and IL-13-induced signaling (). Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 and TRIF through activation of PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:22043923). Inhibits osteoclast formation. Induces macrophage cell death upon engagement ().