Rabbit IL6 Polyclonal Antibody | anti-IL6 antibody

IL6 Polyclonal Antibody

Cat. Num. AAA3007753

• Gene Names: IL6; CDF; HGF; HSF; BSF2; IL-6; BSF-2; IFNB2; IFN-beta-2
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >96% (by SDS-PAGE).
• Synonyms: IL6; Polyclonal Antibody; IL6 Polyclonal Antibody; Interleukin-6; IL-6; B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2; IFNB2; anti-IL6 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IL6 polyclonal antibody detects endogenous levels of IL6 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >96% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
• Sequence Length: 212

• Applicable Applications for anti-IL6 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to Human IL6.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of IL6 polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysate (40ug) Lane2:U-87MG whole cell lysate (40ug) Lane3:HuT78 whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug) Lane5:MCF-7 whole cell lysate (40ug))

Related Product Information for anti-IL6 antibody

Acute phase response is induced by interleukin-6 (IL-6) produced by T cells, macrophages, fibroblasts, endothelial and other cells. IL-6 induces proliferation or differentiation in many cell types including B cells, thymocytes and T cells. IL-6, in concert with TGF-beta, is important for developing Th17 responses. IL-6 binds to IL-6Ralpha and through this association induces gp130 homodimerization. gp130 homodimerization triggers the Jak/Stat cascade and the SHP-2/Erk MAP kinase cascade. IL-6 also forms a complex with an IL-6Ralpha splice variant that is nonmembrane-associated. The IL-6/soluble IL-6Ralpha complex can then activate the gp130 signaling pathway in cells that express gp130 but not IL-6Ralpha. Research studies have shown that IL-6, through increasing expression of proangiogenic VEGF, may also contribute to metastatic breast cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 124347
• NCBI GeneID: 3569
• UniProt Accession #: P05231
• Molecular Weight: ~ 28kDa
• NCBI Official Full Name: Interleukin-6
• NCBI Official Synonym Full Names: interleukin 6
• NCBI Official Symbol: IL6
• NCBI Official Synonym Symbols: CDF; HGF; HSF; BSF2; IL-6; BSF-2; IFNB2; IFN-beta-2
• NCBI Protein Information: interleukin-6
• UniProt Protein Name: Interleukin-6
• Protein Family: Interleukin
• UniProt Gene Name: IL6
• UniProt Synonym Gene Names: IFNB2; IL-6; BSF-2; CDF; IFN-beta-2
• UniProt Entry Name: IL6_HUMAN

NCBI Description

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]