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Western Blot (WB) (Lanes:30ug NIH3T3 cell lysatePrimary Antibody Dilution:1:1000Secondary Antibody:Anti-rabbit HRPSecondary Antibody Dilution:1: 5000Gene Name:Ttf1Submitted by:Thomas Moss)

Rabbit anti-Mouse, Rat Ttf1 Polyclonal Antibody | anti-TTF1 antibody

Ttf1 antibody - N-terminal region

Gene Names
Ttf1; TTF-1; TTF-I; AV245725
Reactivity
Mouse, Rat
Applications
Western Blot
Purity
Affinity Purified
Synonyms
Ttf1; Polyclonal Antibody; Ttf1 antibody - N-terminal region; anti-TTF1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Sequence
Synthetic peptide located within the following region: RRASQTPAQETLESEWPQKAKRKKRRREPQTPAQETLESEWPQKAKKKKR
Sequence Length
859
Applicable Applications for anti-TTF1 antibody
Western Blot (WB)
Homology
Mouse: 100%; Rat: 91%
Immunogen
The immunogen is a synthetic peptide directed towards the n terminal region of mouse Ttf1
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Lanes:30ug NIH3T3 cell lysatePrimary Antibody Dilution:1:1000Secondary Antibody:Anti-rabbit HRPSecondary Antibody Dilution:1: 5000Gene Name:Ttf1Submitted by:Thomas Moss)

Western Blot (WB) (Lanes:30ug NIH3T3 cell lysatePrimary Antibody Dilution:1:1000Secondary Antibody:Anti-rabbit HRPSecondary Antibody Dilution:1: 5000Gene Name:Ttf1Submitted by:Thomas Moss)

Western Blot (WB)

(WB Suggested Anti-Ttf1 Antibody Titration: 0.2-1 ug/mlPositive Control: Mouse Heart)

Western Blot (WB) (WB Suggested Anti-Ttf1 Antibody Titration: 0.2-1 ug/mlPositive Control: Mouse Heart)
Related Product Information for anti-TTF1 antibody
This is a rabbit polyclonal antibody against Ttf1. It was validated on Western Blot using a cell lysate as a positive control.

Target Description: Ttf1 is a multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin.Ttf1 plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription.Ttf1 interaction with BAZ2A/TIP5 recovers DNA-binding activity.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
98
NCBI Official Full Name
transcription termination factor 1
NCBI Official Synonym Full Names
transcription termination factor, RNA polymerase I
NCBI Official Symbol
Ttf1
NCBI Official Synonym Symbols
TTF-1; TTF-I; AV245725
NCBI Protein Information
transcription termination factor 1
UniProt Protein Name
Transcription termination factor 1
UniProt Gene Name
Ttf1
UniProt Synonym Gene Names
TTF-1; TTF-I; mTFF-I

Uniprot Description

Multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. Plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5 recovers DNA-binding activity.

Research Articles on TTF1

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Product Notes

The TTF1 ttf1 (Catalog #AAA3203407) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ttf1 antibody - N-terminal region reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ttf1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the TTF1 ttf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: RRASQTPAQE TLESEWPQKA KRKKRRREPQ TPAQETLESE WPQKAKKKKR. It is sometimes possible for the material contained within the vial of "Ttf1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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