Rabbit anti-Rat IL17F Polyclonal Antibody | anti-IL17F antibody

Anti-IL17F Antibody, Rabbit Polyclonal

Cat. Num. AAA8113033

• Reactivity: Rat
• Applications: Western Blot, ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: IL17F; Polyclonal Antibody; Anti-IL17F Antibody; Rabbit Polyclonal; IL-17F Antibody; Rabbit PAb; Antigen Affinity Purified; interleukin 17F; anti-IL17F antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rat IL-17F
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-IL17F antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000
• Immunogen: Recombinant Rat IL-17F Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Rat IL-17F (rR IL-17F). IL-17F specific IgG was purified by Rat IL-17F affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-ratIL17F rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:18 kDaObserved band size:15 kDa)

Related Product Information for anti-IL17F antibody

Interleukin-17F (IL-17F) is a cytokine that shares sequence similarity with IL17. The most notable role of IL-17 is its involvement in inducing and mediating proinflammatory responses. IL-17 is commonly associated with allergic responses. IL-17F is expressed by activated T cells and was expressed only in activated CD4+ T cells and activated monocytes. IL-17F has been shown to stimulate the production of several other cytokines, including IL6 and IL8. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. Recombinant human IL-17F did not stimulate the proliferation of hematopoietic progenitors or the migration of mature leukocytes. However, it markedly inhibited the angiogenesis of human endothelial cells and induced endothelial cells to produce IL-2, TGF-{beta}, and monocyte chemoattractant protein-1. IL-17F stimulates the production of other cytokines and granulocyte colony-stimulating factor and can regulate cartilage matrix turnover. IL-17F stimulates PBMC and T-cell proliferation. It also functions in inhibiting angiogenesis By similarity. IL-17F plays a role in the induction of neutrophilia in the lungs and the exacerbation of antigen-induced pulmonary allergic inflammation.

Product Categories/Family for anti-IL17F antibody

Neuroinflammation; IL17 signaling pathway

References

et al.. Starnes T, et al.. (2001) Cutting edge: IL-17F, a novel cytokine selectively expressed in activated T cells and monocytes, regulates angiogenesis and endothelial cell cytokine production. J Immunol. 167(8): 4137-40.Hymowitz SG, et al.. (2001) IL-17s adopt a cystine knot fold: structure and activity of a novel cytokine, IL-17F, and implications for receptor binding. EMBO J. 20(19): 5332-41.McAllister F, et al.. (2005) Role of IL-17A, IL-17F, and the IL-17 receptor in regulating growth-related oncogene-alpha and granulocyte colony-stimulating factor in bronchial epithelium: implications for airway inflammation in cystic fibrosis. J Immunol. 175(1): 404-12.

NCBI and Uniprot Product Information

• NCBI GI #: 62543517
• NCBI GeneID: 301291
• NCBI Accession #: NP_001015011.1
• NCBI GenBank Nucleotide #: NM_001015011.1
• UniProt Accession #: Q5BJ95
• Molecular Weight: 17,712 Da
• NCBI Official Full Name: interleukin-17F
• NCBI Official Synonym Full Names: interleukin 17F
• NCBI Official Symbol: Il17f
• NCBI Protein Information: interleukin-17F; IL-17F
• UniProt Protein Name: Interleukin-17F
• Protein Family: Interleukin
• UniProt Gene Name: Il17f
• UniProt Synonym Gene Names: IL-17F
• UniProt Entry Name: IL17F_RAT

Uniprot Description

IL17F: Stimulates the production of other cytokines such as IL- 6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. Stimulates PBMC and T-cell proliferation. Inhibits angiogenesis. Defects in IL17F are the cause of familial candidiasis type 6 (CANDF6). CANDF6 is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans. Belongs to the IL-17 family.

Protein type: Secreted; Secreted, signal peptide; Cytokine

Cellular Component: extracellular space

Molecular Function: protein homodimerization activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine binding; cytokine activity

Biological Process: proteoglycan metabolic process; negative regulation of angiogenesis; regulation of transforming growth factor beta receptor signaling pathway; regulation of interleukin-8 biosynthetic process; lymphotoxin A biosynthetic process; cartilage development; regulation of interleukin-2 biosynthetic process; regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; cytokine biosynthetic process; regulation of interleukin-6 biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; inflammatory response