Rabbit anti-Mouse IL-5 Polyclonal Antibody | anti-IL5 antibody

Anti-IL-5 Antibody, Rabbit Polyclonal

Cat. Num. AAA8112287

• Gene Names: Il5; Il-5
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: IL-5; Polyclonal Antibody; Anti-IL-5 Antibody; Rabbit Polyclonal; IL-5/IL5/Interleukin 5 Antibody; Rabbit PAb; Antigen Affinity Purified; interleukin 5; Anti-Il-5 Antibody; anti-IL5 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse IL-5/IL5/Interleukin 5
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-IL5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000
• Immunogen: Recombinant Mouse IL-5/IL5/Interleukin 5 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse IL-5/IL5/Interleukin 5 (rM IL-5/IL5/Interleukin 5). IL-5/IL5/Interleukin 5 specific IgG was purified by Mouse IL-5/IL5/Interleukin 5 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-IL5 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: HT-29 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:15 kDaObserved band size:18 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-IL5 antibody

Interleukin 5 (IL-5) is a member of the interleukin family with a length of 115 amino acids. Interleukins are a group of cytokines (secreted proteins/signaling molecules) that were first seen to be expressed by white blood cells (leukocytes) and has been found in a wide variety of body cells. Interleukin 5 or IL-5 is produced by T helper-2 cells and mast cells. It helps to stimulate B cell growth and increase immunoglobulin secretion and is considered a key mediator in eosinophil activation. Interleukin 5 (IL-5) has long been associated with several allergic diseases, including allergic rhinitis and asthma. Growth in the number of circulating, airway tissue, and induced sputum eosinophils have been observed in patients with these diseases. IL-5 also had something with the terminally differentiated granulocyte eosinophils. IL-5 was originally found as an eosinophil colony-stimulating factor. It has been proved to be a major regulator of eosinophil accumulation in tissues and can modulate eosinophil behavior at every stage from maturation to survival.

References

Milburn MV, et al. (1993) A novel dimer configuration revealed by the crystal structure at 2.4 A resolution of human interleukin-5. Nature. 363(6425): 172-176.Lee JS, et al. (1989) The IL-4 and IL-5 genes are closely linked and are part of a cytokine gene cluster on mouse chromosome 11. Somat Cell Mol Genet. 15(2): 143-152.Woodcock JM, et al. (1994) Three residues in the common beta chain of the human GM-CSF, IL-3 and IL-5 receptors are essential for GM-CSF and IL-5 but not IL-3 high affinity binding and interact with Glu21 of GM-CSF. EMBO J. 13 (21): 5176-85.

NCBI and Uniprot Product Information

• NCBI GI #: 6754336
• NCBI GeneID: 16191
• NCBI Accession #: NP_034688.1
• NCBI GenBank Nucleotide #: NM_010558.1
• UniProt Accession #: P04401
• Molecular Weight: 15,410 Da
• NCBI Official Full Name: interleukin-5
• NCBI Official Synonym Full Names: interleukin 5
• NCBI Official Symbol: Il5
• NCBI Official Synonym Symbols: Il-5
• NCBI Protein Information: interleukin-5; B-cell growth factor II; BCGF-II; T-cell replacing factor; TRF; cytotoxic T-lymphocyte inducer; eosinophil differentiation factor
• UniProt Protein Name: Interleukin-5
• Protein Family: Interleukin
• UniProt Gene Name: Il5
• UniProt Synonym Gene Names: Il-5; IL-5; BCGF-II; TRF
• UniProt Entry Name: IL5_MOUSE

Uniprot Description

IL5: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells. Belongs to the IL-5 family.

Protein type: Secreted, signal peptide; Cytokine; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function: interleukin-5 receptor binding; growth factor activity; cytokine activity

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation; positive regulation of B cell proliferation; positive regulation of JAK-STAT cascade; immune response; positive regulation of transcription factor activity; positive regulation of immunoglobulin secretion; positive regulation of eosinophil differentiation