CD124 recombinant protein

Recombinant Human CD124 protein, C-His Tag

Cat. Num. AAA1560575

• Gene Names: IL4R; CD124; IL4RA; IL-4RA
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CD124; Recombinant Human CD124 protein; C-His Tag; IL4R; IL4RA; CD124 recombinant protein

• Host: Mammalian cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Tag: C-His Tag
• Species: Homo sapiens (Human)
• Endotoxin Level: < 1.0EU per ug of the protein as determined by the LAL method.
• Protein Construction: A DNA sequence encoding the human IL4R (Met1-His232) was fused with the C-terminal His Tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

SDS-Page

Related Product Information for CD124 recombinant protein

IL-4 is a pleiotropic cytokine produced by activated Th2 cells and mast cells, and plays a pivotal role in immune responses. The effects of IL-4 are mediated after binding to high affinity receptor complexes present on hematopoietic as well as non-hematopoietic cells. Hematopoietic cellular responses to IL-4 are mediated by a high affinity receptor complex comprised of the 140kDa IL4Ralpha (CD124)subunit and the 70kDa common cytokine gammac chain (CD132). Interleukin 4 Receptor (IL4R) also known as CD124, IL4Ralpha and BSF receptor, is a type I cytokine receptor produced by activated Th2 cells and mast cells, and plays an important role in Th2-­biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis. A soluble form of the encoded IL4R protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. IL4R can alternatively associate with IL­13Ra1 to form the type II receptor which is responsive to both IL­4 and IL­13. Interleukin-4 receptor has been shown to interact with SHC1.

Product Categories/Family for CD124 recombinant protein

Protein

References

Han, Bai, Zhou, Niu, Yuan, Zhang, Jia, Zhao, Chen (2020) Dietary supplementation with polysaccharides from Ziziphus Jujuba cv. Pozao intervenes in immune response via regulating peripheral immunity and intestinal barrier function in cyclophosphamide-induced mice Food & function 11(7) 5992-6006

NCBI and Uniprot Product Information

• NCBI GI #: 4557669
• NCBI GeneID: 3566
• NCBI Accession #: NP_000409.1
• NCBI GenBank Nucleotide #: NM_000418.3
• UniProt Accession #: P24394; Q96P01; Q9H181; Q9H182; Q9H183; Q9H184; Q9H185; Q9H186; Q9H187; Q9H188; B9EKU8; H3BSY5
• Molecular Weight: 89,658 Da
• NCBI Official Full Name: interleukin-4 receptor subunit alpha isoform a
• NCBI Official Synonym Full Names: interleukin 4 receptor
• NCBI Official Symbol: IL4R
• NCBI Official Synonym Symbols: CD124; IL4RA; IL-4RA
• NCBI Protein Information: interleukin-4 receptor subunit alpha; IL4R nirs variant 1; IL-4 receptor subunit alpha; interleukin-4 receptor alpha chain
• UniProt Protein Name: Interleukin-4 receptor subunit alpha
• UniProt Gene Name: IL4R
• UniProt Synonym Gene Names: IL4RA; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; Soluble IL-4 receptor subunit alpha; Soluble IL-4R-alpha; sIL4Ralpha/prot; IL4-BP
• UniProt Entry Name: IL4RA_HUMAN

NCBI Description

This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Polymorphisms in this gene are also associated with resistance to human immunodeficiency virus type-1 infection. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012]

Uniprot Description

IL4R: Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2. Belongs to the type I cytokine receptor family. Type 4 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 16p12.1-p11.2

Cellular Component: extracellular space; integral to plasma membrane; receptor complex

Molecular Function: protein binding; interleukin-4 receptor activity; receptor signaling protein activity

Biological Process: ovulation; positive regulation of T-helper 2 cell differentiation; negative regulation of T-helper 1 cell differentiation; response to estrogen stimulus; production of molecular mediator of acute inflammatory response; immune response; defense response to protozoan; signal transduction; regulation of cell proliferation; positive regulation of macrophage activation

Disease: Ige Responsiveness, Atopic; Human Immunodeficiency Virus Type 1, Susceptibility To