Rabbit anti-Mouse, Rat IL-27 RECEPTOR ALPHA Polyclonal Antibody | anti-IL-27 ALPHA antibody

RABBIT ANTI HUMAN IL-27 RECEPTOR ALPHA (C-TERMINAL)

Cat. Num. AAA223375

• Gene Names: IL27RA; CRL1; TCCR; WSX1; IL27R; IL-27RA; zcytor1
• Reactivity: Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Synonyms: IL-27 RECEPTOR ALPHA; Polyclonal Antibody; RABBIT ANTI HUMAN IL-27 RECEPTOR ALPHA (C-TERMINAL); anti-IL-27 ALPHA antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5mg/ml (varies by lot)
• Sequence Length: 636

• Applicable Applications for anti-IL-27 ALPHA antibody: Immunofluorescence (IF), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Western Blot: Rabbit anti human IL-27Ralpha detects a band of approximately 70kDa in human spleen tissue lysates.; ; Immunohistology-Paraffin 5 ug/mL; Western Blotting 0.5-1.0 ug/mL; Immunofluorescence 20 ug/mL; ; This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
• Preservative Stabilisers: 0.02% Sodium Azide (NaN3)
• Buffer Solution: Phosphate buffered saline
• Antiserum Preparation: Antiserum to human IL-27R alpha (C-Terminal) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4°C or at -20°C if preferred. ; ; Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; ; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western Blot analysis of human spleen lysate probed with Rabbit anti Human IL-27 receptor alpha (MBS223375))

Testing Data #2

(Immunohistochemical staining of human spleen with Rabbit anti Human IL-27 receptor alpha (MBS223375))

Related Product Information for anti-IL-27 ALPHA antibody

Rabbit anti human IL-27Ralpha antibody recognizes the carboxy terminal of the alpha subunit of the human IL-27 receptor, otherwise known as the T cell cytokine receptor (TCCR). It is a member of the type I cytokine receptor family with higher levels of expression in CD4+ T cells than in CD8+ T cells. It is a receptor for Interleukin-27 and is involved in the regulation of Th1-type T cells. It also appears to be involved in innate defense mechanisms. It is highly expressed in lymphoid tissue such as spleen, lymph nodes and peripheral blood leukocytes. It is also weakly expressed in tissues such as the heart, brain and lung.

NCBI and Uniprot Product Information

• NCBI GI #: 4759328
• NCBI GeneID: 9466
• NCBI Accession #: NP_004834.1
• NCBI GenBank Nucleotide #: NM_004843.3
• UniProt Accession #: Q6UWB1; O60624; A0N0L1
• NCBI Official Full Name: interleukin-27 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 27 receptor, alpha
• NCBI Official Symbol: IL27RA
• NCBI Official Synonym Symbols: CRL1; TCCR; WSX1; IL27R; IL-27RA; zcytor1
• NCBI Protein Information: interleukin-27 receptor subunit alpha; IL-27 receptor subunit alpha; IL-27R subunit alpha; IL-27R-alpha; T-cell cytokine receptor type 1; class I cytokine receptor; cytokine receptor WSX-1; cytokine receptor-like 1; type I T-cell cytokine receptor
• UniProt Protein Name: Interleukin-27 receptor subunit alpha
• UniProt Gene Name: IL27RA
• UniProt Synonym Gene Names: CRL1; TCCR; WSX1; IL-27 receptor subunit alpha; IL-27R subunit alpha; IL-27R-alpha; IL-27RA; TCCR
• UniProt Entry Name: I27RA_HUMAN

NCBI Description

In mice, CD4+ helper T-cells differentiate into type 1 (Th1) cells, which are critical for cell-mediated immunity, predominantly under the influence of IL12. Also, IL4 influences their differentiation into type 2 (Th2) cells, which are critical for most antibody responses. Mice deficient in these cytokines, their receptors, or associated transcription factors have impaired, but are not absent of, Th1 or Th2 immune responses. This gene encodes a protein which is similar to the mouse T-cell cytokine receptor Tccr at the amino acid level, and is predicted to be a glycosylated transmembrane protein. [provided by RefSeq, Jul 2008]

Uniprot Description

IL27RA: Receptor for IL27. Requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1- type immune responses. Also appears to be involved in innate defense mechanisms. Belongs to the type I cytokine receptor family. Type 2 subfamily.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: integral to plasma membrane

Molecular Function: transmembrane receptor activity; interleukin-27 receptor activity

Biological Process: defense response to Gram-positive bacterium; positive regulation of interferon-gamma production; cell surface receptor linked signal transduction; positive regulation of T-helper 1 type immune response; negative regulation of T-helper 2 type immune response; immune response; regulation of isotype switching to IgG isotypes