Goat anti-Mouse IL-23 RECEPTOR Polyclonal Antibody | anti-IL23R antibody

GOAT ANTI HUMAN INTERLEUKIN-23 RECEPTOR (N-TERMINAL)

Cat. Num. AAA223501

• Reactivity: Mouse
• Applications: Immunohistochemistry, Western Blot
• Synonyms: IL-23 RECEPTOR; Polyclonal Antibody; GOAT ANTI HUMAN INTERLEUKIN-23 RECEPTOR (N-TERMINAL); anti-IL23R antibody

• Host: Goat
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 629

• Applicable Applications for anti-IL23R antibody: Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Immunohistology - Paraffin: Minimum Dilution: 1/125; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections; Western Blotting: Minimum Dilution: 1/500
• Perservative Stabilisers: 0.1% Sodium Azide (NaN3); 0.1% Bovine Serum Albumin
• Histology Positive Control Tissue: Spleen; Buffer Solution
• Antiserum Preparation: Antiserum to human IL-23R (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Paraffin embedded human spleen stained with Goat anti human IL-23 Receptor (MBS223501))

Testing Data #2

(Paraffin embedded mouse spleen stained with Goat anti human IL-23 Receptor (MBS223501))

Related Product Information for anti-IL23R antibody

Goat anti Human Interleukin-23 receptor antibody recognizes the human interleukin-23 receptor (IL-23R). The interleukin-23 receptor is 629 amino acid ~72kDa single pass type I transmembrane glycoprotein belonging to the type I cytokine receptor family and posessing two fibronectin type III domains. Multiple potential isoforms formed by alternative splicing are described. Goat anti Human Interleukin-23 receptor antibody recognizes an epitope within the N-terminal (NT) region of Interleukin-23 receptor (IL-23R), precsent in isoforms 1, 3 and 4 (UniProt: Q5VWK5), the anytibody is not expected to recognize other truncated forms of the receptor lacking the immunogen sequence. IL-23R is a mediator of T-cell, NK cell, and possibly macrophage/myeloid cell, stimulation acting as the receptor for IL-23, by association with the beta component of IL-12R and is thought to signal through activation of the JAK-STAT signalling pathway. Genetic variation in IL23R is associated with inflammatory bowel disease type 17 (Kistner et al. 2006) and susceptibility to the ulcerative and inflammatory psoriasis (Hüffmeier et al. 2009). Goat anti Human Interleukin-23 receptor antibody is reported as suitable for use in immunocytochemistry on acetone fixed cells and has been used successfully for the immunohistochemical localization of IL-23R in formalin fixed, paraffin embedded human tissues..

NCBI and Uniprot Product Information

• NCBI GI #: 24430212
• NCBI GeneID: 149233
• NCBI Accession #: NP_653302.2
• NCBI GenBank Nucleotide #: NM_144701.2
• Molecular Weight: 25,551 Da
• NCBI Official Full Name: interleukin-23 receptor
• NCBI Official Synonym Full Names: interleukin 23 receptor
• NCBI Official Symbol: IL23R
• NCBI Protein Information: interleukin-23 receptor; IL-23 receptor
• UniProt Protein Name: Interleukin-23 receptor
• Protein Family: Interleukin-23 receptor
• UniProt Gene Name: IL23R
• UniProt Synonym Gene Names: IL-23 receptor; IL-23R
• UniProt Entry Name: IL23R_HUMAN

NCBI Description

The protein encoded by this gene is a subunit of the receptor for IL23A/IL23. This protein pairs with the receptor molecule IL12RB1/IL12Rbeta1, and both are required for IL23A signaling. This protein associates constitutively with Janus kinase 2 (JAK2), and also binds to transcription activator STAT3 in a ligand-dependent manner. [provided by RefSeq, Jul 2008]

Uniprot Description

IL23R: Associates with IL12RB1 to form the interleukin-23 receptor. Binds IL23 and mediates T-cells, NK cells and possibly certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade. IL23 functions in innate and adaptive immunity and may participate in acute response to infection in peripheral tissues. IL23 may be responsible for autoimmune inflammatory diseases and be important for tumorigenesis. Genetic variations in IL23R are associated with inflammatory bowel disease type 17 (IBD17). IBD17 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Genetic variations in IL23R are a cause of susceptibility to psoriasis type 7 (PSORS7). Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis. Belongs to the type I cytokine receptor family. Type 2 subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: receptor complex

Molecular Function: interleukin-23 receptor activity; interleukin-23 binding; interleukin-12 receptor binding

Biological Process: positive regulation of memory T cell differentiation; positive regulation of interleukin-17 production; positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of NK T cell activation; positive regulation of natural killer cell proliferation; positive regulation of T-helper 1 type immune response; negative regulation of interleukin-10 production; positive regulation of interleukin-12 production; positive regulation of T cell mediated cytotoxicity; positive regulation of osteoclast differentiation; positive regulation of tyrosine phosphorylation of Stat4 protein; response to lipopolysaccharide; defense response to Gram-negative bacterium; positive regulation of activated T cell proliferation; regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of interferon-gamma production; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of T cell proliferation; positive regulation of defense response to virus by host; inflammatory response

Disease: Inflammatory Bowel Disease 17; Psoriasis 7, Susceptibility To