Rabbit anti-Mouse IL-20 Polyclonal Antibody | anti-IL20 antibody

Anti-IL-20 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100395

• Gene Names: Il20; Zcyto10
• Reactivity: Mouse
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: IL-20; Polyclonal Antibody; Anti-IL-20 Antibody; Rabbit Polyclonal; IL20 Antibody; Rabbit PAb; Antigen Affinity Purified; interleukin 20; Anti-Zcyto10 Antibody; anti-IL20 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse IL20
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-IL20 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IP: 1-2uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the center region of the mouse mIL20
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with A synthetic peptide corresponding to the center region of the mouse mIL20, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-IL20 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:20 kDaObserved band size:18 kDa(We are unsure as to the identity of these extra bands.))

Immunoprecipitation (IP)

(IL20 was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell LysateLane B:0.5 mg K562 Whole Cell Lysate0.5 uL anti-IL20 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-IL20 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 18 kDaObserved band size: 20 kDa)

Related Product Information for anti-IL20 antibody

IL20/Interleukin-20 belongs to the IL-10 family. It is a cytokine structurally related to interleukin 10. IL20/Interleukin-20 can be detected in skin, trachea, and other tissues. It is produced by activated keratinocytes and monocytes and transmits an intracellular signal through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells. It has been shown that interleukin-20 transduces its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. It may be involved in epidermal function and psoriasis. It also regulates the proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. Also, IL20/Interleukin-20 also causes cell expansion of multipotential hematopoietic progenitor cells. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis.

References

Chen XY, et al. (2011) The association between the IL-20-1723CG allele on the 1q chromosome and psoriasis triggered or exacerbated by an upper respiratory tract infection in the Chinese Han population. Dermatology. 222(1):24-30.Baird AM, et al. (2011) IL-20 is epigenetically regulated in NSCLC and down regulates the expression of VEGF. Eur J Cancer. 47(12):1908-18.Logsdon NJ, et al. (2012) Purification, crystallization and preliminary X-ray diffraction analysis of the IL-20-IL-20R1-IL-20R2 complex. Acta Crystallogr Sect F Struct Biol Cryst Commun. 68(Pt 1):89-92.

NCBI and Uniprot Product Information

• NCBI GI #: 10946716
• NCBI GeneID: 58181
• NCBI Accession #: NP_067355.1
• NCBI GenBank Nucleotide #: NM_021380.1
• UniProt Accession #: Q9JKV9
• Molecular Weight: 20,098 Da
• NCBI Official Full Name: interleukin-20
• NCBI Official Synonym Full Names: interleukin 20
• NCBI Official Symbol: Il20
• NCBI Official Synonym Symbols: Zcyto10
• NCBI Protein Information: interleukin-20; IL-20; cytokine Zcyto10; four alpha helix cytokine ZCYTO10; four helix bundle cytokine 10
• UniProt Protein Name: Interleukin-20
• Protein Family: Interleukin
• UniProt Gene Name: Il20
• UniProt Synonym Gene Names: Zcyto10; IL-20
• UniProt Entry Name: IL20_MOUSE

Uniprot Description

IL20: Cytokine that may be involved in epidermal function and psoriasis. Acts through STAT3. Belongs to the IL-10 family.

Protein type: Secreted, signal peptide; Secreted; Cytokine

Cellular Component: extracellular space; cell; extracellular region; intracellular

Molecular Function: interleukin-20 receptor binding; cytokine activity; interleukin-22 receptor binding; receptor binding

Biological Process: positive regulation of osteoclast differentiation; positive regulation of JAK-STAT cascade; hemopoiesis; immune response; inflammatory response; STAT protein nuclear translocation