Rabbit anti-Human IL-1RAcP Polyclonal Antibody | anti-IL1RAP antibody

IL-1RAcP Antibody

Cat. Num. AAA150885

• Gene Names: IL1RAP; IL1R3; C3orf13; IL-1RAcP
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: IL-1RAcP Antibody is affinity chromatography purified via peptide column.
• Synonyms: IL-1RAcP; Polyclonal Antibody; IL-1RAcP Antibody; IL1R3; C3orf13; Interleukin-1 receptor accessory protein; Interleukin-1 receptor 3; IL-1 receptor accessory protein; interleukin 1 receptor accessory protein; anti-IL1RAP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: It has no cross activity to other member in the IL-1 receptor family.
• Purity/Purification: IL-1RAcP Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Sequence Length: 1740

• Applicable Applications for anti-IL1RAP antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: IL-1RAcP antibody can be used for detection of IL-1RAcP by Western blot 0.5 mug/mL. An approximately 66 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 mug/mL.
• Conjugate: Unconjugated
• Immunogen: IL-1RAcP antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of IL-1RAcP, which differs from. The sequence of mouse origin by three amino acids.
• Buffer: IL-1RAcP Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: IL-1RAcP antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of IL-1RAcP expression in K562 cell lysate with IL-1RAcP antibody at 1 μg/ml.)

Immunocytochemistry (IHC)

(Immunocytochemistry of IL-1RAcP in HeLa cells with IL-1RAcP antibody at 5 μg/mL.)

Related Product Information for anti-IL1RAP antibody

IL-1RAcP Antibody: The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1 receptor I(IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 RAcP forms a complex with IL-1RI in response to IL-1 treatment. The IL-1 receptor-associated kinase (IRAK), which mediates activation of NF-κB inducing kinae (NIK) and of NF-κB, recruites to the IL-1R complex through IL-1 RAcP. IL-1 activation of stress-activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP. Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 2599126
• NCBI GeneID: 3556
• UniProt Accession #: Q9NPH3; O14915; Q86WJ7; B1NLD0; D3DNW0
• Molecular Weight: 66 kDa
• NCBI Official Full Name: Homo sapiens IL-1 receptor accessory protein mRNA, complete cds
• NCBI Official Synonym Full Names: interleukin 1 receptor accessory protein
• NCBI Official Symbol: IL1RAP
• NCBI Official Synonym Symbols: IL1R3; C3orf13; IL-1RAcP
• NCBI Protein Information: interleukin-1 receptor accessory protein; IL-1R3; interleukin-1 receptor 3; IL-1 receptor accessory protein; interleukin-1 receptor accessory protein beta
• UniProt Protein Name: Interleukin-1 receptor accessory protein
• Protein Family: Interleukin-1 receptor accessory protein
• UniProt Gene Name: IL1RAP
• UniProt Synonym Gene Names: C3orf13; IL1R3; IL-1 receptor accessory protein; IL-1RAcP; IL-1R-3; IL-1R3
• UniProt Entry Name: IL1AP_HUMAN

NCBI Description

Interleukin 1 induces synthesis of acute phase and proinflammatory proteins during infection, tissue damage, or stress, by forming a complex at the cell membrane with an interleukin 1 receptor and an accessory protein. This gene encodes the interleukin 1 receptor accessory protein. The protein is a necessary part of the interleukin 1 receptor complex which initiates signalling events that result in the activation of interleukin 1-responsive genes. Alternative splicing of this gene results in two transcript variants encoding two different isoforms, one membrane-bound and one soluble. The ratio of soluble to membrane-bound forms increases during acute-phase induction or stress. [provided by RefSeq, Nov 2009]

Uniprot Description

IL1RAP: Coreceptor with IL1R1. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Secreted forms (isoforms 2 and 3) associate with secreted ligand-bound IL1R2 and increase the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. Belongs to the interleukin-1 receptor family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: membrane; integral to plasma membrane; plasma membrane; extracellular region

Molecular Function: signal transducer activity; interleukin-1 receptor activity

Biological Process: interleukin-2 biosynthetic process; cytokine and chemokine mediated signaling pathway; innate immune response; protein complex assembly; immune response; inflammatory response