Human Exosome Component 7 (EXOSC7) ELISA Kit | EXOSC7 elisa kit

Human Exosome Component 7 (EXOSC7) ELISA Kit

Cat. Num. AAA2707087

• Gene Names: EXOSC7; p8; EAP1; RRP42; Rrp42p; hRrp42p
• Reactivity: Human
• Synonyms: Exosome Component 7 (EXOSC7); Human Exosome Component 7 (EXOSC7) ELISA Kit; Rrp42p; RRP42; EAP1; p8; hRrp42p; Ribosomal RNA-processing protein 42; Exosome complex component RRP42; EXOSC7 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Exosome Component 7 (EXOSC7).; No significant cross-reactivity or interference between Exosome Component 7 (EXOSC7) and analogues was observed.
• Sequence Length: 291

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.065ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Exosome Component 7 (EXOSC7) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Exosome Component 7 (EXOSC7) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EXOSC7 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Exosome Component 7 (EXOSC7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Exosome Component 7 (EXOSC7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Exosome Component 7 (EXOSC7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Exosome Component 7 (EXOSC7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 15215485
• NCBI GeneID: 23016
• Molecular Weight: 31,821 Da
• NCBI Official Full Name: Exosome component 7
• NCBI Official Synonym Full Names: exosome component 7
• NCBI Official Symbol: EXOSC7
• NCBI Official Synonym Symbols: p8; EAP1; RRP42; Rrp42p; hRrp42p
• NCBI Protein Information: exosome complex component RRP42
• UniProt Protein Name: Exosome complex component RRP42
• Protein Family: Exosome complex exonuclease
• UniProt Gene Name: EXOSC7
• UniProt Synonym Gene Names: KIAA0116; RRP42

Uniprot Description

Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes.