Western Blot (WB)
(Fig.1. Western blot analysis of Mouse-kidney lysate, antibody was diluted at 1:1000. HRP, Goat Anti-Rabbit IgG secondary antibody was diluted at 1:20000.)
Rabbit anti-Human IL-12B Polyclonal Antibody | anti-IL-12B antibody
IL-12B p40 Polyclonal Antibody
Gene Names
IL12B; CLMF; NKSF; CLMF2; IMD28; IMD29; NKSF2; IL-12B
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Synonyms
IL-12B; Polyclonal Antibody; IL-12B p40 Polyclonal Antibody; Rabbit Anti-IL-12B p40 Polyclonal Antibody; Interleukin-12 subunit beta; Cytotoxic lymphocyte maturation factor 40 kDa subunit; CLMF p40; IL-12 subunit p40; NK cell stimulatory factor chain 2; NKSF2; anti-IL-12B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
The antibody detects endogenous levels of IL-12B p40
Purity/Purification
Affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form/Format
Liquid; PBS, pH 7.4, Containing 0.02% Sodium Azide As Preservative and 50% Glycerol As Stabilizer.
Concentration
1 mg/ml (varies by lot)
Sequence Length
328
Applicable Applications for anti-IL-12B antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:500-1:200
ELISA: 1:10000-1:20000
IHC-P: 1:500-1:200
ELISA: 1:10000-1:20000
Immunogen
Synthesized peptide derived from part region of human IL-12B p40 protein at amino acid sequence of 241-290
Preparation and Storage
Stable for one year at -20 degree C from date of shipment.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Western Blot (WB)
(Fig.1. Western blot analysis of Mouse-kidney lysate, antibody was diluted at 1:1000. HRP, Goat Anti-Rabbit IgG secondary antibody was diluted at 1:20000.)
Western Blot (WB)
(Fig.1. Western blot analysis of Mouse-kidney lysate, antibody was diluted at 1:1000. HRP, Goat Anti-Rabbit IgG secondary antibody was diluted at 1:20000.)
Immunohistochemistry (IHC)
(Fig.2. Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200.)
Immunohistochemistry (IHC)
(Fig.2. Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200.)
Immunohistochemistry (IHC)
(Fig.3. Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200.)
Immunohistochemistry (IHC)
(Fig.3. Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200.)
Related Product Information for anti-IL-12B antibody
IL12B encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by IL12B, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of IL12B was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of IL12B has been reported to be associated with the severity of atopic and non-atopic asthma in children.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
interleukin-12 subunit beta
NCBI Official Synonym Full Names
interleukin 12B
NCBI Official Symbol
IL12B
NCBI Official Synonym Symbols
CLMF; NKSF; CLMF2; IMD28; IMD29; NKSF2; IL-12B
NCBI Protein Information
interleukin-12 subunit beta
UniProt Protein Name
Interleukin-12 subunit beta
UniProt Gene Name
IL12B
UniProt Synonym Gene Names
NKSF2; IL-12B; CLMF p40; NKSF2
UniProt Entry Name
IL12B_HUMAN
NCBI Description
This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. [provided by RefSeq, Jul 2008]
Research Articles on IL-12B
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Product Notes
The IL-12B il12b (Catalog #AAA9709479) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IL-12B p40 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-12B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA). WB: 1:500-1:2000 IHC-P: 1:500-1:200 ELISA: 1:10000-1:20000. Researchers should empirically determine the suitability of the IL-12B il12b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-12B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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