Rabbit IL-10Ralpha  Polyclonal Antibody | anti-IL-10Ralpha  antibody

IL-10Ralpha (Phospho-Y496) Polyclonal Antibody

Cat. Num. AAA3005078

• Gene Names: IL10RA; CD210; IL10R; CD210a; CDW210A; HIL-10R; IL-10R1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: IL-10Ralpha ; Polyclonal Antibody; IL-10Ralpha (Phospho-Y496) Polyclonal Antibody; IL-10R-A; IL-10R1; CDw210a antigen; IL10RA; IL10R; anti-IL-10Ralpha antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-IL-10Ralpha (Y496) pAb detects endogenous levels of IL-10Ralpha protein only when phosphorylated at Tyr496.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 578

• Applicable Applications for anti-IL-10Ralpha antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic phosphopeptide derived from human IL-10Ralpha around the phosphorylation site of Tyrosine 496
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of p-IL-10Ralpha  (Y496) pAb at 1:500 dilutionLane1:Myla2059 whole cell lysate (40ug) Lane2:K562 whole cell lysate (40ug) Lane3:RAW264. 7 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug))

Related Product Information for anti-IL-10Ralpha  antibody

The IL-10 receptor, IL-10R, is a member of the class II subgroup of the cytokine receptor family and exhibits structural similarity to the interferon receptor. IL-10R is expressed in B cells and T helper cells, as well as in LPS-induced mouse fibroblasts. Overall, mouse IL-10R and human IL-10R share 60% sequence identity at the protein level. Stimulation with IL-10 leads to phosphorylation of JAK1 and Tyk 2 tyrosine kinases. The activated kinases phosphorylate the two tyrosine residues (Tyr 446 and Tyr 496) in the cytoplasmic domain of IL-10Ralpha. The phosphorylation of these two residues are required for proper function of IL-10R and activation of IL-10E1 signaling. IL-10Rbeta is ubiquitously expressed and, in addition to forming the IL-10 heterodimeric receptor, it forms a heterodimeric receptor with an IL-22R subunit and an IL-28R subunit. IL-10R is constitutively expressed on human natural killer (NK) cells and the direct binding of IL-10 potentiates cytokine production by human NK cells.

NCBI and Uniprot Product Information

• NCBI GI #: 222136575
• NCBI GeneID: 3587
• NCBI Accession #: NP_001549.2
• NCBI GenBank Nucleotide #: NP_001549.2
• UniProt Accession #: Q13651
• Molecular Weight: ~ 70kDa
• NCBI Official Full Name: interleukin-10 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 10 receptor subunit alpha
• NCBI Official Symbol: IL10RA
• NCBI Official Synonym Symbols: CD210; IL10R; CD210a; CDW210A; HIL-10R; IL-10R1
• NCBI Protein Information: interleukin-10 receptor subunit alpha
• UniProt Protein Name: Interleukin-10 receptor subunit alpha
• UniProt Gene Name: IL10RA
• UniProt Synonym Gene Names: IL10R; IL-10 receptor subunit alpha; IL-10R subunit alpha; IL-10RA; IL-10R subunit 1; IL-10R1
• UniProt Entry Name: I10R1_HUMAN

NCBI Description

The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2009]

Uniprot Description

IL10RA: a receptor for interleukin 10 structurally related to interferon receptors. Mediates the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: plasma membrane; integral to membrane

Molecular Function: signal transducer activity; protein binding; interleukin-10 binding; interleukin-10 receptor activity; receptor activity

Biological Process: cytokine and chemokine mediated signaling pathway; response to lipopolysaccharide

Disease: Inflammatory Bowel Disease 28, Autosomal Recessive