Rabbit IKKB Polyclonal Antibody | anti-IKBKB antibody

IKKB, ID (IKBKB, IKKB, Inhibitor of light polypeptide kappa-B kinase subunit beta, I-kappa-B kinase 2)

Cat. Num. AAA6007973

• Gene Names: IKBKB; IKK2; IKKB; NFKBIKB; IKK-beta
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography
• Synonyms: IKKB; Polyclonal Antibody; ID (IKBKB; Inhibitor of light polypeptide kappa-B kinase subunit beta; I-kappa-B kinase 2); Anti -IKKB; anti-IKBKB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IKBKB/IKK. Species Crossreactivity: Mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography
• Form/Format: Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-IKBKB antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA and Immunohistochemistry.; Dilution: ELISA: 1:10,000; Immunohistochemistry (Paraffn): 10ug/ml
• Immunogen: Synthetic peptide corresponding to human IKBKB/IKK.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(IKBKB/IKK Beta IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced retrieval.)

Related Product Information for anti-IKBKB antibody

IKKbeta, an IKAPPAB -type protein kinase, is a member of the multimeric IkappaB kinase complex. This complex, often consisting of IKKalpha/IKKbeta heterodimers plus regulatory IKKgamma subunits, phosphorylates IkappaB proteins, marking them for destruction and thereby allowing activation of NFkappaB and downstream nuclear genes. The constitutively expressed IKKbeta protein contains an N-terminal kinase domain, a central leucine zipper motif, and a C-terminal helix-loop-helix motif. In knockout studies, IKKbeta (-/-) mice died between embryonic day 12.5 and 14 due to extensive liver damage from apoptosis; however, they were rescued by inactivation of the tumor necrosis factor receptor 1 gene. IKKbeta has been implicated in the pathogenesis of insulin resistance in obesity and type II diabetes mellitus.

Product Categories/Family for anti-IKBKB antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 338753358
• NCBI GeneID: 3551
• NCBI Accession #: NP_001229707.1
• NCBI GenBank Nucleotide #: NM_001242778.1
• UniProt Accession #: O14920; O75327; B4DZ30; B4E0U4
• Molecular Weight: 86,564 Da
• NCBI Official Full Name: inhibitor of nuclear factor kappa-B kinase subunit beta isoform 5
• NCBI Official Synonym Full Names: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
• NCBI Official Symbol: IKBKB
• NCBI Official Synonym Symbols: IKK2; IKKB; NFKBIKB; IKK-beta
• NCBI Protein Information: inhibitor of nuclear factor kappa-B kinase subunit beta; IKK-B; I-kappa-B kinase 2; I-kappa-B-kinase beta; nuclear factor NF-kappa-B inhibitor kinase beta
• UniProt Protein Name: Inhibitor of nuclear factor kappa-B kinase subunit beta
• UniProt Gene Name: IKBKB
• UniProt Synonym Gene Names: IKKB; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; IKK2; NFKBIKB
• UniProt Entry Name: IKKB_HUMAN

NCBI Description

The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

IKKB: a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Preferentially found as a heterodimer with IKK-alpha but also as an homodimer.

Protein type: Protein kinase, Other; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.10; Other group; IKK family

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: internal side of plasma membrane; cytoplasm; IkappaB kinase complex; cytosol; nucleus; lipid raft

Molecular Function: protein serine/threonine kinase activity; protein binding; protein homodimerization activity; IkappaB kinase activity; protein heterodimerization activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; response to virus; positive regulation of transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; T cell receptor signaling pathway; toll-like receptor 2 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; serine phosphorylation of STAT protein; B cell homeostasis; positive regulation of interferon type I production; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; I-kappaB phosphorylation; inflammatory response; toll-like receptor 4 signaling pathway; negative regulation of apoptosis

Disease: Immunodeficiency 15