Rabbit anti-Mouse IKKB Polyclonal Antibody | anti-IKKB antibody

IKKB, Phosphorylated (S701) (Ikbkb, Ikkb, Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B kinase 2, Nuclear factor NF-kappa-B inhibitor kinase beta) (MaxLight 550)

Cat. Num. AAA6309768

• Gene Names: Ikbkb; IKK2; IKK-2; IKK[b]; IKKbeta; AI132552; IKK-beta
• Reactivity: Mouse
• Applications: Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IKKB; Polyclonal Antibody; Phosphorylated (S701) (Ikbkb; Ikkb; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B kinase 2; Nuclear factor NF-kappa-B inhibitor kinase beta) (MaxLight 550); Ikbkb; Nuclear factor NF-kappa-B inhibitor kinase beta; anti-IKKB antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-IKKB antibody: FLISA, Dot Blot (DB)
• Application Notes: FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: mouse IKKB Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S701 of mouse IKKB.
• Conjugate: MaxLight550
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-IKKB antibody

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).

Product Categories/Family for anti-IKKB antibody

Antibodies; Protein Kinases

References

Busuttil, V., et al. Proc. Natl. Acad. Sci. U.S.A. 107(42):18061-18066(2010). Kenneth, N.S., et al. EMBO J. 29(17):2966-2978(2010). Tsuchiya, Y., et al. Mol. Cell 39(4):570-582(2010). Farlik, M., et al. Immunity 33(1):25-34(2010). Dong, X., et al. PLoS Pathog. 6 (7), E1001001 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 33469101
• NCBI GeneID: 16150
• NCBI Accession #: NP_034676.1
• NCBI GenBank Nucleotide #: NM_010546.2
• UniProt Accession #: O88351; Q9R1J6
• Molecular Weight: 86,690 Da
• NCBI Official Full Name: inhibitor of nuclear factor kappa-B kinase subunit beta isoform 2
• NCBI Official Synonym Full Names: inhibitor of kappaB kinase beta
• NCBI Official Symbol: Ikbkb
• NCBI Official Synonym Symbols: IKK2; IKK-2; IKK[b]; IKKbeta; AI132552; IKK-beta
• NCBI Protein Information: inhibitor of nuclear factor kappa-B kinase subunit beta
• UniProt Protein Name: Inhibitor of nuclear factor kappa-B kinase subunit beta
• UniProt Gene Name: Ikbkb
• UniProt Synonym Gene Names: Ikkb; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; IKK2; NFKBIKB

Uniprot Description

Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation.