Rabbit anti-Human, Mouse IkappaB-alpha (Phospho-Tyr42) Antibody Polyclonal Antibody | anti-NFKBIA antibody

IkappaB-alpha (Phospho-Tyr42) Antibody

Cat. Num. AAA9414086

• Gene Names: NFKBIA; IKBA; MAD-3; NFKBI
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• Synonyms: IkappaB-alpha (Phospho-Tyr42) Antibody; Polyclonal Antibody; IkB-a (Phospho-Tyr42) Antibody; IKBA; MAD-3; NFKBI; NFKBIA; anti-NFKBIA antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of IkB-a only when phosphorylated at tyrosine 42.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 317

• Applicable Applications for anti-NFKBIA antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500~1:1000; Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of tyrosine 42 (E-E-Y(p)-E-Q) derived from Human IkB-a
• Modification: Phospho
• Target Name: IkB-a
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extract from Hela cells untreated or treated with serum using IkB-a(Phospho-Tyr42) antibody (MBS9414086))

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-a(phospho-Tyr42) (MBS9414086))

Related Product Information for anti-NFKBIA antibody

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.

Product Categories/Family for anti-NFKBIA antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 10092619
• NCBI GeneID: 4792
• NCBI Accession #: NP_065390.1
• NCBI GenBank Nucleotide #: NM_020529.2
• UniProt Accession #: P25963; B2R8L6
• Molecular Weight: Predicted MW: 38kD
• NCBI Official Full Name: NF-kappa-B inhibitor alpha
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• NCBI Official Symbol: NFKBIA
• NCBI Official Synonym Symbols: IKBA; MAD-3; NFKBI
• NCBI Protein Information: NF-kappa-B inhibitor alpha
• UniProt Protein Name: NF-kappa-B inhibitor alpha
• Protein Family: NF-kappa-B inhibitor
• UniProt Gene Name: NFKBIA
• UniProt Synonym Gene Names: IKBA; MAD3; NFKBI; IkB-alpha; IkappaBalpha
• UniProt Entry Name: IKBA_HUMAN

NCBI Description

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Uniprot Description

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.