Rabbit anti-Human IkappaB-alpha Polyclonal Antibody | anti-IkappaB-alpha antibody

IkappaB-alpha (Ab-32/36) Antibody

Cat. Num. AAA9405294

• Gene Names: NFKBIA; IKBA; MAD-3; NFKBI
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: IkappaB-alpha; Polyclonal Antibody; IkappaB-alpha (Ab-32/36) Antibody; IkB-a (Ab-32/36) Antibody; I-kappa-B-alpha; IkappaBalpha; IKBA; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; anti-IkappaB-alpha antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total IkB-a protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 317

• Applicable Applications for anti-IkappaB-alpha antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500~1:3000; Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide
• Immunogen Description: Synthesized non-phosphopeptide derived from human IkB-a around the phosphorylation site of Serine 32/36.
• Target Name: IkB-a
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from MCF7 cells, treated with TNF-a, using IkappaB-alpha (Ab-32/36) antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using IkappaB-alpha (Ab-32/36) antibody.)

Related Product Information for anti-IkappaB-alpha antibody

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.Mattioli I, et al. (2004)J Immunol; 172(10): 6336-44.Courtois G, et al. (2003)J Clin Invest; 112(7): 1108-15.Nair A, et al. (2003) Oncogene; 22(1): 50-8.Fan C, et al. (2002)J Cell Sci; 115(Pt 24): 4843-53.Schubert SY, et al. (2002)FASEB J; 16(14): 193

Product Categories/Family for anti-IkappaB-alpha antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 10092619
• NCBI GeneID: 4792
• NCBI Accession #: NP_065390.1
• NCBI GenBank Nucleotide #: NM_020529.2
• UniProt Accession #: P25963; B2R8L6
• Molecular Weight: 35,609 Da
• NCBI Official Full Name: NF-kappa-B inhibitor alpha
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• NCBI Official Symbol: NFKBIA
• NCBI Official Synonym Symbols: IKBA; MAD-3; NFKBI
• NCBI Protein Information: NF-kappa-B inhibitor alpha
• UniProt Protein Name: NF-kappa-B inhibitor alpha
• UniProt Gene Name: NFKBIA
• UniProt Synonym Gene Names: IKBA; MAD3; NFKBI; IkB-alpha; IkappaBalpha
• UniProt Entry Name: IKBA_HUMAN

NCBI Description

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Uniprot Description

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.