Goat anti-Mouse IgG, IgA, IgM, H&L, X-adsorbed Polyclonal Antibody
IgG, IgA, IgM, H&L, X-adsorbed (Biotin)
Reactivity
Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
IgG; IgA; IgM; H&L; X-adsorbed; Polyclonal Antibody; X-adsorbed (Biotin); Anti -IgG; anti-IgG; X-adsorbed antibody
Host
Goat
Reactivity
Mouse
Clonality
Polyclonal
Isotype
F(ab')2
Specificity
Recognizes Mouse IgG, IgA and IgM, whole molecules (all subclasses, isotypes and chain combinations). Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-goat serum. Species Crossreactivity: Does not crossreact with human serum protein.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Reconstitute with 1ml sterile, distilled, deionized water or sterile 40-50% glycerol for long-term storage.. Labeled with Biotin.
Applicable Applications for anti-IgG, IgA, IgM, H&L, X-adsorbed antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications not tested
Dilution: ELISA: 1:5000-1:20,000
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:500-1:2000
Optimal dilution to be determined by the researcher.
Dilution: ELISA: 1:5000-1:20,000
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:500-1:2000
Optimal dilution to be determined by the researcher.
Immunogen
Mouse IgG, IgA and IgM whole molecules
Label
Biotinamidocaproate N-Hydroxysuccinimide Ester(BAC)
Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule
Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Product Categories/Family for anti-IgG, IgA, IgM, H&L, X-adsorbed antibody
Similar Products
Product Notes
The IgG, IgA, IgM, H&L, X-adsorbed (Catalog #AAA616651) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The IgG, IgA, IgM, H&L, X-adsorbed (Biotin) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IgG, IgA, IgM, H&L, X-adsorbed can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications not tested Dilution: ELISA: 1:5000-1:20,000 Western Blot: 1:500-1:2000 Immunohistochemistry: 1:500-1:2000 Optimal dilution to be determined by the researcher. Researchers should empirically determine the suitability of the IgG, IgA, IgM, H&L, X-adsorbed for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG, IgA, IgM, H&L, X-adsorbed, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
