Rabbit IGFBP5 Polyclonal Antibody | anti-IGFBP5 antibody

IGFBP5 Antibody

Cat. Num. AAA9607230

• Gene Names: IGFBP5; IBP5
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: IGFBP5; Polyclonal Antibody; IGFBP5 Antibody; IBP 5; IBP-5; IBP5; IBP5_HUMAN; IGF binding protein 5; IGF BP5; IGF-binding protein 5; IGFBP 5; IGFBP-5; Insulin like growth factor binding protein 5; Insulin-like growth factor-binding protein 5; anti-IGFBP5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IGFBP5 antibody detects endogenous levels of total IGFBP5
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 272

• Applicable Applications for anti-IGFBP5 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; ELISA (peptide): 1:20000-1:40000 ; ; IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
• Immunogen: A synthesized peptide derived from human IGFBP5, corresponding to a region within the internal amino acids.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(MBS9607230 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis of extracts from PC12, using IGFBP5 antibody. The lane on the left is treated with blocking peptide.)

Related Product Information for anti-IGFBP5 antibody

Description: IGFBP5, also named as IBP5, prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. It alter the interaction of IGFs with their cell surface receptors. This antibody is specific to IGFBL5.

NCBI and Uniprot Product Information

• NCBI GI #: 10834982
• NCBI GeneID: 3488
• NCBI Accession #: NP_000590.1
• NCBI GenBank Nucleotide #: NM_000599.3
• UniProt Accession #: P24593; Q5U0A3
• Molecular Weight: 31 kDa
• NCBI Official Full Name: insulin-like growth factor-binding protein 5
• NCBI Official Synonym Full Names: insulin like growth factor binding protein 5
• NCBI Official Symbol: IGFBP5
• NCBI Official Synonym Symbols: IBP5
• NCBI Protein Information: insulin-like growth factor-binding protein 5
• UniProt Protein Name: Insulin-like growth factor-binding protein 5
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: IGFBP5
• UniProt Synonym Gene Names: IBP5; IBP-5; IGF-binding protein 5; IGFBP-5

Uniprot Description

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.