Rabbit anti-Human, Mouse IGFALS Polyclonal Antibody | anti-IGFALS antibody

IGFALS (Insulin-like Growth Factor Binding Protein Complex Acid Labile Subunit, ALS) (HRP)

Cat. Num. AAA6481419

• Gene Names: IGFALS; ALS; ACLSD
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: IGFALS; Polyclonal Antibody; IGFALS (Insulin-like Growth Factor Binding Protein Complex Acid Labile Subunit; ALS) (HRP); Insulin-like Growth Factor-binding Protein; Acid Labile Subunit; anti-IGFALS antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IGFALS. Species Crossreactivity: mouse
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-IGFALS antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-1:50; WB: 1:1000; IHC-P: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 308-335 in the central region of human IGFALS, UniProt Accession #P35858
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western blot analysis in mouse spleen tissue, Jurkat cell line and mouse liver tissue lysates (35ug/lane) usingMBS630019. IGFALS (arrow) was detected usingMBS630019.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MBS630019 (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow cytometry analysis of Jurkat cells (bottom histogram) using MBS630019 compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-IGFALS antibody

Antibodies; Serum Proteins

References

1. Leong S.R., Baxter R.C.Mol. Endocrinol. 6:870-876(1992). 2. Suwanichkul A.Endocrinology 141:833-838(2000). 3. Liu T., Qian W.-J.J. Proteome Res. 4:2070-2080(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 4826772
• NCBI GeneID: 3483
• NCBI Accession #: NP_004961
• NCBI GenBank Nucleotide #: NM_004970
• UniProt Accession #: P35858
• Molecular Weight: 63kDa
• NCBI Official Full Name: insulin-like growth factor-binding protein complex acid labile subunit isoform 2
• NCBI Official Synonym Full Names: insulin like growth factor binding protein acid labile subunit
• NCBI Official Symbol: IGFALS
• NCBI Official Synonym Symbols: ALS; ACLSD
• NCBI Protein Information: insulin-like growth factor-binding protein complex acid labile subunit
• UniProt Protein Name: Insulin-like growth factor-binding protein complex acid labile subunit
• Protein Family: Insulin-like growth factor-binding protein complex acid labile
• UniProt Gene Name: IGFALS
• UniProt Synonym Gene Names: ALS; ALS
• UniProt Entry Name: ALS_HUMAN

NCBI Description

The protein encoded by this gene is a serum protein that binds insulin-like growth factors, increasing their half-life and their vascular localization. Production of the encoded protein, which contains twenty leucine-rich repeats, is stimulated by growth hormone. Defects in this gene are a cause of acid-labile subunit deficiency, which maifests itself in a delayed and slow puberty. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

IGFALS: Involved in protein-protein interactions that result in protein complexes, receptor-ligand binding or cell adhesion.

Protein type: Cell adhesion; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; extracellular space; extracellular region

Molecular Function: insulin-like growth factor binding

Biological Process: cellular protein metabolic process; signal transduction; cell adhesion

Disease: Acid-labile Subunit Deficiency