Rabbit anti-Human, Mouse IGF2R Polyclonal Antibody | anti-IGF2R antibody

IGF2R (Phospho-Ser2409) Antibody

Cat. Num. AAA9406982

• Gene Names: IGF2R; MPR1; MPRI; CD222; CIMPR; M6P-R
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: IGF2R; Polyclonal Antibody; IGF2R (Phospho-Ser2409) Antibody; CI-MPR; MPR300; MPRI; anti-IGF2R antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of IGF2R only when phosphorylated at serine 2409.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 2491

• Applicable Applications for anti-IGF2R antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500~1:1000; Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of Serine 2409(Q-D-S(p)-E-D) derived from Human IGF2R.
• Modification: Phospho-Ser2409
• Target Name: IGF2R
• Preparation and Storage: Store at -20 degree C/1 year

Western Blot (WB)

(Western blot analysis of extracts from COS-7 cells treated with UV using IGF2R (Phospho-Ser2409) Antibody.The lane on the right is treated with the antigen-specific peptide.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human brain tissue using IGF2R (Phospho-Ser2409) antibody (left)or the same antibody preincubated with blocking peptide (right).)

Related Product Information for anti-IGF2R antibody

Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.Morgan D.O., Nature 329:301-307(1987).Oshima A., J. Biol. Chem. 263:2553-2562(1988).Killian J.K., Mamm. Genome 10:74-77(1999)

Product Categories/Family for anti-IGF2R antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 119964726
• NCBI GeneID: 3482
• NCBI Accession #: NP_000867.2
• NCBI GenBank Nucleotide #: NM_000876.2
• UniProt Accession #: P11717; Q7Z7G9; Q96PT5
• Molecular Weight: 274,375 Da
• NCBI Official Full Name: cation-independent mannose-6-phosphate receptor
• NCBI Official Synonym Full Names: insulin-like growth factor 2 receptor
• NCBI Official Symbol: IGF2R
• NCBI Official Synonym Symbols: MPR1; MPRI; CD222; CIMPR; M6P-R
• NCBI Protein Information: cation-independent mannose-6-phosphate receptor
• UniProt Protein Name: Cation-independent mannose-6-phosphate receptor
• Protein Family: Cation-independent mannose-6-phosphate receptor
• UniProt Gene Name: IGF2R
• UniProt Synonym Gene Names: MPRI; CI Man-6-P receptor; CI-MPR; M6PR; MPR 300; IGF-II receptor; M6P/IGF2R
• UniProt Entry Name: MPRI_HUMAN

NCBI Description

This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate, although the binding sites for either are located on different segments of the receptor. This receptor functions in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. While the related mouse gene shows exclusive expression from the maternal allele, imprinting of the human gene appears to be polymorphic, with only a minority of individuals showing expression from the maternal allele. [provided by RefSeq, Apr 2013]

Uniprot Description

IGF2R: a multifunctional type I transmembrane protein receptor that binds insulin-like growth factor 2 at the cell surface and mannose-6-phosphate-tagged proteins in the trans-Golgi network. Also known as the cation-independent mannose-6 phosphate (M6P) receptor. Clears IGF2 from the cell surface to attenuate signaling, and transports M6P-tagged lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. M6P-tagged lysosomal enzymes bind specifically to mannose-6- phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Belongs to the MRL1/IGF2R family.

Protein type: Membrane protein, integral; Vesicle; Receptor, misc.

Chromosomal Location of Human Ortholog: 6q26

Cellular Component: extracellular space; trans-Golgi network transport vesicle; clathrin coat; focal adhesion; cell surface; membrane; lysosomal membrane; integral to plasma membrane; nuclear envelope lumen; endocytic vesicle; perinuclear region of cytoplasm; late endosome; trans-Golgi network; endosome

Molecular Function: G-protein coupled receptor activity; mannose binding; identical protein binding; protein binding; retinoic acid binding; enzyme binding; transporter activity; G-protein alpha-subunit binding; phosphoprotein binding; receptor activity; glycoprotein binding; insulin-like growth factor II binding; insulin-like growth factor receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; receptor-mediated endocytosis; organ regeneration; response to retinoic acid; insulin-like growth factor receptor signaling pathway; positive regulation of apoptosis; spermatogenesis; signal transduction; liver development; post-embryonic development

Disease: Hepatocellular Carcinoma