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Western Blot (WB) (Western blot analysis of lysate from HeLa cells, using IgA antibody.)

Rabbit anti-Human IgA Polyclonal Antibody | anti-IgA antibody

IgA Antibody

Gene Names
IGHA1; IgA1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Synonyms
IgA; Polyclonal Antibody; IgA Antibody; Ig alpha-1 chain C region; Ig alpha-2 chain C region; IGHA1; IGHA2; anti-IgA antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
IgA antibody detects endogenous levels of IgA.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
353
Applicable Applications for anti-IgA antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:10000
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human IgA.
Immunogen Range
227-276
Preparation and Storage
Stable at -20 degree C for at least 1 year.

Western Blot (WB)

(Western blot analysis of lysate from HeLa cells, using IgA antibody.)

Western Blot (WB) (Western blot analysis of lysate from HeLa cells, using IgA antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of IgA antibody in paraffin-embedded human lung carcinoma tissue.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of IgA antibody in paraffin-embedded human lung carcinoma tissue.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
37 kDa
NCBI Official Full Name
Immunoglobulin heavy constant alpha 1
NCBI Official Synonym Full Names
immunoglobulin heavy constant alpha 1
NCBI Official Symbol
IGHA1
NCBI Official Synonym Symbols
IgA1
UniProt Protein Name
Immunoglobulin heavy constant alpha 1
Protein Family
UniProt Gene Name
IGHA1

Uniprot Description

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). Ig alpha is the major immunoglobulin class in body secretions (PubMed:2241915).

Research Articles on IgA

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Product Notes

The IgA igha1 (Catalog #AAA9508885) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IgA Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IgA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000. Researchers should empirically determine the suitability of the IgA igha1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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