Rabbit IFN-gammaRalpha Polyclonal Antibody | anti-IFNRalpha antibody

IFN-gammaRalpha (Phospho-Y457) Polyclonal Antibody

Cat. Num. AAA3005189

• Gene Names: IFNGR1; CD119; IFNGR; IMD27A; IMD27B
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: IFN-gammaRalpha; Polyclonal Antibody; IFN-gammaRalpha (Phospho-Y457) Polyclonal Antibody; Interferon gamma receptor 1; IFN-gamma receptor 1; IFN-gamma-R1; CDw119; CD119; IFNGR1; anti-IFNRalpha antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-IFN-gammaRalpha (Y457) polyclonal antibody detects endogenous levels of IFN-gammaRalpha protein only when phosphorylated at Tyr457.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 489

• Applicable Applications for anti-IFNRalpha antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic phosphopeptide derived from human IFN-gammaRalpha around the phosphorylation site of Tyrosine 457.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of p-IFNGR1 (Y457) polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysate treated with IFN-gamma (100ug/ML,15mins) Lane2:HNIH-3T3 cell lysate treated with IFN-gamma (100ug/ML,15mins) Lane3:H9C2 cell lysate treated with IFN-gamma (100ug/ML,15mins))

Related Product Information for anti-IFNRalpha antibody

IFN gamma receptor beta is part of the receptor for interferon gamma. This class II cytokine receptor pairs with CDw119 to form the IFN gamma receptor and is an integral part of the IFN gamma signal transduction pathway. CDw119 serves as the IFN gamma binding chain and associates with the IFN gamma beta chain which is required for receptor signaling. The extracellular portion of both the IFN gamma receptor alpha and beta chains must be species matched. The IFN gamma receptor beta chain is expressed on T and B cells, NK cells, monocytes/ macrophages, and fibroblasts.

NCBI and Uniprot Product Information

• NCBI GI #: 4557880
• NCBI GeneID: 3459
• NCBI Accession #: NP_000407.1
• NCBI GenBank Nucleotide #: NP_000407.1
• UniProt Accession #: P15260
• Molecular Weight: ~ 54, 90kDa
• NCBI Official Full Name: interferon gamma receptor 1 isoform 1
• NCBI Official Synonym Full Names: interferon gamma receptor 1
• NCBI Official Symbol: IFNGR1
• NCBI Official Synonym Symbols: CD119; IFNGR; IMD27A; IMD27B
• NCBI Protein Information: interferon gamma receptor 1
• UniProt Protein Name: Interferon gamma receptor 1
• UniProt Gene Name: IFNGR1
• UniProt Synonym Gene Names: IFN-gamma receptor 1
• UniProt Entry Name: INGR1_HUMAN

NCBI Description

This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection. [provided by RefSeq, Jul 2008]

Uniprot Description

IFNGR1: Receptor for interferon gamma. Two receptors bind one interferon gamma dimer. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD); also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance. Belongs to the type II cytokine receptor family.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 6q23.3

Cellular Component: endoplasmic reticulum; integral to plasma membrane; dendrite; postsynaptic density; integral to membrane; plasma membrane; vesicle

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; interferon-gamma receptor activity; cytokine binding

Biological Process: cytokine and chemokine mediated signaling pathway; response to virus; signal transduction

Disease: Helicobacter Pylori Infection, Susceptibility To; Immunodeficiency 27b; Immunodeficiency 27a; Mycobacterium Tuberculosis, Susceptibility To; Hepatitis B Virus, Susceptibility To