Rabbit anti-Human ENT1 Polyclonal Antibody | anti-ENT1 antibody

ENT1, Phosphorylated (Ser254) (Equilibrative Nitrobenzylmercaptopurine Riboside-sensitive Nucleoside Transporter, Equilibrative NBMPR-sensitive Nucleoside Transporter,Nucleoside Transporter, es-type, Solute Carrier Family 29 Member 1, Slc29a1, Equilibrati

Cat. Num. AAA6474098

• Reactivity: Human
• Applications: ELISA, Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ENT1; Polyclonal Antibody; Phosphorylated (Ser254) (Equilibrative Nitrobenzylmercaptopurine Riboside-sensitive Nucleoside Transporter; Equilibrative NBMPR-sensitive Nucleoside Transporter; Nucleoside Transporter; es-type; Solute Carrier Family 29 Member 1; Slc29a1; Equilibrati; anti-ENT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ENT1 when phosphorylated at Ser254.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ENT1 antibody: ELISA (EIA), Dot Blot (DB)
• Application Notes: ELISA: 1:1,000; Dot Blot: 1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser254 of human ENT1 (KLH).
• Conjugate: HRP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-Phospho-ENT1(Slc29a1)-pS254 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Product Categories/Family for anti-ENT1 antibody

Antibodies; Transport Proteins

References

1. Dephoure N, et al. (2008). Proc Natl Acad Sci USA 105, 10762-7. 2. Bone DB, Robillard KR, Stolk M, Hammond JR (2007). Mol Membr Biol 24, 294-303.

NCBI and Uniprot Product Information

• NCBI GI #: 118582260
• UniProt Accession #: Q99808
• Molecular Weight: 50.219
• UniProt Protein Name: Equilibrative nucleoside transporter 1
• Protein Family: Epsin
• UniProt Gene Name: SLC29A1
• UniProt Synonym Gene Names: ENT1; Equilibrative NBMPR-sensitive nucleoside transporter
• UniProt Entry Name: S29A1_HUMAN

Uniprot Description

SLC29A1: Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs). Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.

Protein type: Membrane protein, integral; Transporter, SLC family; Membrane protein, multi-pass; Transporter

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: membrane; integral to plasma membrane; basolateral plasma membrane; apical plasma membrane; plasma membrane

Molecular Function: nucleoside transmembrane transporter activity

Biological Process: lactation; uridine transport; nucleoside transport; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; regulation of excitatory postsynaptic membrane potential; sleep; transmembrane transport