Rabbit anti-Human IBSP Polyclonal Antibody | anti-IBSP antibody

IBSP, NT (IBSP, BNSP, Bone sialoprotein 2, Bone sialoprotein II, Cell-binding sialoprotein, Integrin-binding sialoprotein) (Azide free) (HRP)

Cat. Num. AAA6309039

• Gene Names: IBSP; BSP; BNSP; SP-II; BSP-II
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IBSP; Polyclonal Antibody; NT (IBSP; BNSP; Bone sialoprotein 2; Bone sialoprotein II; Cell-binding sialoprotein; Integrin-binding sialoprotein) (Azide free) (HRP); Integrin-binding sialoprotein; anti-IBSP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IBSP.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-IBSP antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 70-99 in the N-terminal region of human IBSP, UniProt Accession #NP_004958.2; P21815.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in CEM cell line lysates (35ug/lane) usingMBS6012925. This demonstrates that MBS6012925 detected the IBSP protein (arrow).)

Related Product Information for anti-IBSP antibody

The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.

Product Categories/Family for anti-IBSP antibody

Antibodies; Integrin

References

1. Giroux, S., et al. Bone 47(5):975-981(2010). 2. Koller, D.L., et al. J. Clin. Endocrinol. Metab. 95(4):1802-1809(2010). 3. Yerges, L.M., et al. J. Bone Miner. Res. 4(12):2039-2049(2009). 4. Gordon, J.A., et al. J. Cell. Biochem. 107(6):1118-1128(2009). 5. Hwang, Q., et al. BMC Cancer 9, 121 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 167466187
• NCBI GeneID: 3381
• NCBI Accession #: NP_004958.2
• NCBI GenBank Nucleotide #: NM_004967.3
• UniProt Accession #: P21815
• Molecular Weight: 35,148 Da
• NCBI Official Full Name: bone sialoprotein 2
• NCBI Official Synonym Full Names: integrin-binding sialoprotein
• NCBI Official Symbol: IBSP
• NCBI Official Synonym Symbols: BSP; BNSP; SP-II; BSP-II
• NCBI Protein Information: bone sialoprotein 2; BSP II; bone sialoprotein II; cell-binding sialoprotein
• UniProt Protein Name: Bone sialoprotein 2
• Protein Family: Bone sialoprotein
• UniProt Gene Name: IBSP
• UniProt Synonym Gene Names: BNSP; BSP II
• UniProt Entry Name: SIAL_HUMAN

NCBI Description

The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

Sialoprotein-2: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q21.1

Cellular Component: extracellular space; membrane; extracellular region

Biological Process: osteoblast differentiation; extracellular matrix organization and biogenesis; biomineral formation; cell adhesion