Rabbit anti-Human I2BP2 Polyclonal Antibody | anti-I2BP2 antibody

I2BP2, ID (IRF2BP2, Interferon regulatory factor 2-binding protein 2) (AP)

Cat. Num. AAA6309013

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: I2BP2; Polyclonal Antibody; ID (IRF2BP2; Interferon regulatory factor 2-binding protein 2) (AP); IRF2BP2; Interferon regulatory factor 2-binding protein 2; anti-I2BP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-I2BP2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: I2BP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 344-374 amino acids from the Central region of human I2BP2.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(I2BP2 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the I2BP2 antibody detected the I2BP2 protein (arrow).)

Flow Cytometry (FC/FACS)

(I2BP2 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-I2BP2 antibody

IRF2BP2 encodes an interferon regulatory factor-2 (IRF2) binding protein that interacts with the C-terminal transcriptional repression domain of IRF2. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq].

Product Categories/Family for anti-I2BP2 antibody

Antibodies; Interferon

References

Teng, A.C., et al. FASEB J. (2010). In press: Peng, Y.B., et al. Anim. Genet. 37(6):600-601(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006). Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004). Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 116734704
• NCBI GeneID: 359948
• NCBI Accession #: NP_892017.2; NP_001070865.1
• NCBI GenBank Nucleotide #: NM_182972.2
• UniProt Accession #: Q7Z5L9; Q6P083; Q7Z5L8; Q8N351; Q8WUH8; B1AM35; B1AM36
• Molecular Weight: 17,139 Da
• NCBI Official Full Name: interferon regulatory factor 2-binding protein 2 isoform A
• NCBI Official Synonym Full Names: interferon regulatory factor 2 binding protein 2
• NCBI Official Symbol: IRF2BP2
• NCBI Protein Information: interferon regulatory factor 2-binding protein 2; IRF-2-binding protein 2
• UniProt Protein Name: Interferon regulatory factor 2-binding protein 2
• UniProt Gene Name: IRF2BP2
• UniProt Synonym Gene Names: IRF-2-binding protein 2; IRF-2BP2
• UniProt Entry Name: I2BP2_HUMAN

NCBI Description

This gene encodes an interferon regulatory factor-2 (IRF2) binding protein that interacts with the C-terminal transcriptional repression domain of IRF2. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

IRF2BP2: Acts as a transcriptional corepressor in a IRF2- dependent manner; this repression is not mediated by histone deacetylase activities. Represses the NFAT1-dependent transactivation of NFAT-responsive promoters. Acts as a coactivator of VEGFA expression in cardiac and skeletal muscle. Belongs to the IRF2BP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1q42.3

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: metal ion binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent