Rabbit I-TRAF Polyclonal Antibody | anti-I-TRAF antibody

I-TRAF Antibody

Cat. Num. AAA9505861

• Gene Names: TANK; ITRAF; TRAF2; I-TRAF
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Synonyms: I-TRAF; Polyclonal Antibody; I-TRAF Antibody; ITRAF; TRAF family member-associated NF-kappa-B activator; TRAF-interacting protein; anti-I-TRAF antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: I-TRAF Antibody detects endogenous levels of total I-TRAF protein.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 119

• Applicable Applications for anti-I-TRAF antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:10000
• Target Modification: Unmodified/Total
• Immunogen: The antiserum was produced against synthesized peptide derived from human TANK.
• Immunogen Range: 171-220
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Western Blot (WB)

(Western blot analysis of lysates from HT29 cells, using I-TRAF Antibody. The lane on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 312433953
• NCBI GeneID: 10010
• NCBI Accession #: NP_001186064.1
• NCBI GenBank Nucleotide #: NM_001199135.1
• UniProt Accession #: Q92844; Q7Z4J6; Q92885; D3DPB5
• Molecular Weight: 47 kDa
• NCBI Official Full Name: TRAF family member-associated NF-kappa-B activator isoform a
• NCBI Official Synonym Full Names: TRAF family member associated NFKB activator
• NCBI Official Symbol: TANK
• NCBI Official Synonym Symbols: ITRAF; TRAF2; I-TRAF
• NCBI Protein Information: TRAF family member-associated NF-kappa-B activator
• UniProt Protein Name: TRAF family member-associated NF-kappa-B activator
• UniProt Gene Name: TANK
• UniProt Synonym Gene Names: ITRAF; TRAF2; I-TRAF

NCBI Description

The TRAF (tumor necrosis factor receptor-associated factor) family of proteins associate with and transduce signals from members of the tumor necrosis factor receptor superfamily. The protein encoded by this gene is found in the cytoplasm and can bind to TRAF1, TRAF2, or TRAF3, thereby inhibiting TRAF function by sequestering the TRAFs in a latent state in the cytoplasm. For example, the protein encoded by this gene can block TRAF2 binding to LMP1, the Epstein-Barr virus transforming protein, and inhibit LMP1-mediated NF-kappa-B activation. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

Adapter protein involved in I-kappa-B-kinase (IKK) regulation which constitutively binds TBK1 and IKBKE playing a role in antiviral innate immunity. Acts as a regulator of TRAF function by maintaining them in a latent state. Blocks TRAF2 binding to LMP1 and inhibits LMP1-mediated NF-kappa-B activation. Negatively regulates NF-kappaB signaling and cell survival upon DNA damage (PubMed:25861989). Plays a role as an adapter to assemble ZC3H12A, USP10 in a deubiquitination complex which plays a negative feedback response to attenuate NF-kappaB activation through the deubiquitination of IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage (PubMed:25861989). Promotes UBP10-induced deubiquitination of TRAF6 in response to DNA damage (PubMed:25861989). May control negatively TRAF2-mediated NF-kappa-B activation signaled by CD40, TNFR1 and TNFR2.