Rabbit anti-Mouse, Rat Hypoxia Inducible Factor 2a Polyclonal Antibody | anti-Hif3a antibody

Hypoxia Inducible Factor 2a (Hypoxia Inducible Factor 2 alpha, Hypoxia Inducible Factor 2 alpha Subunit, HIF-2a, Endothelial PAS Domain Containing Protein 1, Endothelial Pas Domain Protein 1, EPAS1, EPAS-1, HIF-2 alpha, HIF2a, HIF1 alpha-like Factor, HLF,

Cat. Num. AAA630666

• Gene Names: Hif3a; Mop7
• Reactivity: Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Hypoxia Inducible Factor 2a; Polyclonal Antibody; Hypoxia Inducible Factor 2a (Hypoxia Inducible Factor 2 alpha; Hypoxia Inducible Factor 2 alpha Subunit; HIF-2a; Endothelial PAS Domain Containing Protein 1; Endothelial Pas Domain Protein 1; EPAS1; EPAS-1; HIF-2 alpha; HIF2a; HIF1 alpha-like Factor; HLF; ; Anti -Hypoxia Inducible Factor 2a (Hypoxia Inducible Factor 2 alpha; anti-Hif3a antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat HIF-2a. Species Cross-Reactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.

• Applicable Applications for anti-Hif3a antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, and Immunocytochemistry.; Dilution: Western Blot: 1ug/ml; Immunohistochemistry: 0.5-1ug/ml
• Immunogen: Synthetic peptide mapping at the aa202-240 of rat HIF-2a.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Hif3a antibody

HIF-2 alpha is also designated EPAS1 whose gene is mapped to 2p21-p16. The predicted mouse protein is 88% identical to human EPAS1. The human EPAS1 gene contains 15 exons and spans at least 120 kb. The positions of the introns within the genomic region encoding the N-terminal bHLH-PAS domains of EPAS1 and AHR are similar, suggesting that the 5-prime ends of the 2 genes may have arisen from a gene duplication event. Moreover, the predicted protein shares 48% sequence identity with HIF1-alpha, a bHLH-PAS transcription factor that induces EPO gene expression in cultured cells in response to hypoxia. Like HIF1A, EPAS1 binds to and activates transcription from the HIF1A response element derived from the 3-prime flanking region of the EPO gene. EPAS1 is predominantly expressed in highly vascularized tissues of adult humans and in endothelial cells of the mouse adult and embryo. Furthermore, EPAS1 may represent an important regulator of vascularization, perhaps involving the regulation of endothelial cell gene expression in response to hypoxia. HIF2A is expressed at relatively higher levels in villus sections of placenta and in lung samples compared with other tissues examined3. In addition, The variation in EPAS1 influences the relative contribution of aerobic and anaerobic metabolism and hence the maximum sustainable metabolic power for a given event duration.

Product Categories/Family for anti-Hif3a antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 11968112
• NCBI GeneID: 64345
• NCBI Accession #: NP_071973.1
• NCBI GenBank Nucleotide #: NM_022528.2
• UniProt Accession #: Q9JHS2
• Molecular Weight: 72,888 Da
• NCBI Official Full Name: hypoxia-inducible factor 3-alpha
• NCBI Official Synonym Full Names: hypoxia inducible factor 3, alpha subunit
• NCBI Official Symbol: Hif3a
• NCBI Official Synonym Symbols: Mop7
• NCBI Protein Information: hypoxia-inducible factor 3-alpha; HIF3-alpha; HIF-3 alpha; HIF-3-alpha; HIF3 alpha 1; HIF3-alpha-1; hypoxia inducible factor 3a; hypoxia inducible factor 3 alpha; hypoxia-inducible factor 3 alpha
• UniProt Protein Name: Hypoxia-inducible factor 3-alpha
• Protein Family: Hypoxia-inducible factor
• UniProt Gene Name: Hif3a
• UniProt Synonym Gene Names: HIF-3-alpha; HIF3-alpha
• UniProt Entry Name: HIF3A_RAT

NCBI Description

binds HIF-responsive elements and activates transcription in vitro [RGD, Feb 2006]

Uniprot Description

HIF3A: Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Tumor suppressor

Cellular Component: cytoplasm; nucleus

Molecular Function: protein dimerization activity; signal transducer activity; DNA binding; transcription coactivator activity; transcription corepressor activity; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; transcription, DNA-dependent; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; signal transduction