Rabbit anti-Human, Mouse Hypoxia Inducible Factor 2a Polyclonal Antibody | anti-Hif3a antibody

Hypoxia Inducible Factor 2a (HIF-2a, EPAS1)

Cat. Num. AAA611017

• Gene Names: Hif3a; Mop7
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Hypoxia Inducible Factor 2a; Polyclonal Antibody; Hypoxia Inducible Factor 2a (HIF-2a; EPAS1); Anti -Hypoxia Inducible Factor 2a (HIF-2a; anti-Hif3a antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Hypoxia Inducible Factor-2 alpha (HIF2a). Species sequence homology: human (100%), rat (92%) and chicken (80%).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, before the addition of glycerol to 40%.

• Applicable Applications for anti-Hif3a antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1-10ug/ml using Chemiluminescence technique; ELISA: 0.5-1ug/ml, use control peptide at 1ug/ml, H9810-01A.
• Immunogen: Synthetic peptide corresponding to15aa of mouse HIF-2a (KLH.). Epitope: Mid-region.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Hif3a antibody

HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis, the posttranslational modification by prolyl hydroxylation as a key regulatory event that targets HIF-alpha subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. The transcriptional complex is composed of an alpha-beta heterodimer; HIF-beta being a constitutive nuclear protein that dimerises with oxygen regulated HIF-alpha subunits.  In normoxia, 4-hydroxylation of human HIF-alpha at Pro402 or Pro564 by a set of HIF prolyl hydroxylase isoenzymes (PHD 1-3) mediates HIF1-alpha recognition by von Hippel-Lindau ubiquitin ligase complex leading to its proteasomal destruction. In hypoxia (deprivation of oxygen), lack of hydroxylase activity enables HIF-alpha subunits to escape destruction and become transcriptionally active. Thus HIF hydroxylases provide a focus for understanding cellular responses to hypoxia and target for therapeutic manipulation. There are several HIF factors, which include HIF 1-alpha, HIF 1-beta, HIF 2-alpha. HIF 2-alpha/ EPAS 1, is a 874aa each protein in mouse, rat and 870aa in human. The transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Expressed in most tissues, with highest levels in lung, followed by heart, kidney, brain and liver.

Product Categories/Family for anti-Hif3a antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 11968112
• NCBI GeneID: 64345
• NCBI Accession #: NP_071973.1
• NCBI GenBank Nucleotide #: NM_022528.2
• UniProt Accession #: Q9JHS2
• Molecular Weight: 72,888 Da
• NCBI Official Full Name: hypoxia-inducible factor 3-alpha
• NCBI Official Synonym Full Names: hypoxia inducible factor 3, alpha subunit
• NCBI Official Symbol: Hif3a
• NCBI Official Synonym Symbols: Mop7
• NCBI Protein Information: hypoxia-inducible factor 3-alpha; HIF3-alpha; HIF-3 alpha; HIF-3-alpha; HIF3 alpha 1; HIF3-alpha-1; hypoxia inducible factor 3a; hypoxia inducible factor 3 alpha; hypoxia-inducible factor 3 alpha
• UniProt Protein Name: Hypoxia-inducible factor 3-alpha
• Protein Family: Hypoxia-inducible factor
• UniProt Gene Name: Hif3a
• UniProt Synonym Gene Names: HIF-3-alpha; HIF3-alpha
• UniProt Entry Name: HIF3A_RAT

NCBI Description

binds HIF-responsive elements and activates transcription in vitro [RGD, Feb 2006]

Uniprot Description

HIF3A: Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Tumor suppressor

Cellular Component: cytoplasm; nucleus

Molecular Function: protein dimerization activity; signal transducer activity; DNA binding; transcription coactivator activity; transcription factor activity; transcription corepressor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; transcription, DNA-dependent; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; signal transduction