Western Blot (WB)
(Western Blot: Sample: Mouse Brain Tissue.)
Rabbit Hypoxia Inducible Factor 2 Alpha (HIF2a) Polyclonal Antibody | anti-HIF2a antibody
Polyclonal Antibody to Hypoxia Inducible Factor 2 Alpha (HIF2a)
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition
Western Blot (WB)
(Western Blot: Sample: Mouse Brain Tissue.)
Western Blot (WB)
(Western Blot: Sample: Mouse Brain Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant HIF2a, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant HIF2a, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Lymph Node Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Lymph Node Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Pancreas Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Pancreas Tissue.)
NCBI and Uniprot Product Information
Uniprot Description
HIF2A: Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerizes with ARNT. Interacts with CREBBP. Interacts with EGLN1. Interacts with VHL. Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 17|17 E4
Cellular Component: cytoplasm; nuclear speck; nucleus; transcription factor complex
Molecular Function: cobalt ion binding; DNA binding; DNA binding transcription factor activity; histone acetyltransferase binding; protein binding; protein dimerization activity; protein heterodimerization activity; sequence-specific DNA binding; transcription factor binding
Biological Process: angiogenesis; blood vessel remodeling; cell differentiation; cell maturation; cellular response to hypoxia; embryonic placenta development; erythrocyte differentiation; hemopoiesis; iron ion homeostasis; lung development; mitochondrion organization; multicellular organism development; myoblast cell fate commitment; negative regulation of transcription from RNA polymerase II promoter; norepinephrine biosynthetic process; norepinephrine metabolic process; positive regulation of transcription from RNA polymerase II promoter; regulation of heart rate; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; regulation of transcription, DNA-templated; response to hypoxia; response to oxidative stress; surfactant homeostasis; transcription, DNA-dependent; visual perception
Research Articles on HIF2a
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Product Notes
The HIF2a epas1 (Catalog #AAA2003798) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Hypoxia Inducible Factor 2 Alpha (HIF2a) reacts with Mus musculus (Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's Hypoxia Inducible Factor 2 Alpha (HIF2a) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, Immunoprecipitation (IP), Western Blot (WB) . Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the HIF2a epas1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hypoxia Inducible Factor 2 Alpha (HIF2a), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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