Rabbit HYPF Polyclonal Antibody | anti-HYPF antibody

Rabbit anti-Escherichia coli (strain K12) HYPF Polyclonal Antibody

• Gene Names: hypF; ECK2707; hydA; JW5433
• Reactivity: E Coli (strain K12)
• Applications: ELISA, Western Blot
• Purity: Protein A/G
• Synonyms: HYPF; Polyclonal Antibody; Rabbit anti-Escherichia coli (strain K12) HYPF Polyclonal Antibody; HYPF Antibody; Carbamoyltransferase HypF; EC 2.1.3.-; Carbamoyl phosphate-converting enzyme HypF; [NiFe]-hydrogenase maturation factor HypF; Hydrogenase maturation protein HypF; hypF hydA b2712 JW5433; anti-HYPF antibody

• Host: Rabbit
• Reactivity: E Coli (strain K12)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A/G
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH7.4

• Applicable Applications for anti-HYPF antibody: ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
• Immunogen: Recombinant Escherichia coli (strain K12) hypF protein
• Conjugation: Non-conjugate
• Modification: Unmodified
• Deliverables: 1. 200ug recombinant immunogen protein/peptide (positive control).; 2. 1mL preimmune serum (negative control); 3. 10mg rabbit polyclonal antibody purified by Protein A/G.
• Quality Guarantee: 1. Antibody purity >90% confirmed by SDS-PAGE.; 2. Antibody titer > 1: 64,000 confirmed by ELISA; 3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
• Complementary Antibody: Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:; * 6*His Monoclonal Antibody #MBS1491670; * DYKDDDDK tag Monoclonal Antibody #MBS1493946; * GAPDH Monoclonal Antibody #MBS1495524; * GFP Monoclonal Antibody #MBS1496050; * Myc tag Monoclonal Antibody #MBS1497694; * GST Monoclonal Antibody #MBS1497871; * Sumo tag Monoclonal Antibody #MBS1498878; * Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510; * HA-Tag Monoclonal Antibody #MBS7135631; * E-Tag Monoclonal Antibody #MBS7135632; * TUBA1A Monoclonal Antibody #MBS7135645; * TUBB Monoclonal Antibody #MBS9018332
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

• NCBI GI #: 16130619
• NCBI GeneID: 944963
• NCBI Accession #: NP_417192.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P30131; Q2MAB7; Q46878
• Molecular Weight: 82,066 Da
• NCBI Official Full Name: carbamoyl phosphate phosphatase and
• NCBI Official Symbol: hypF
• NCBI Official Synonym Symbols: ECK2707; hydA; JW5433
• NCBI Protein Information: carbamoyl phosphate phosphatase and [NiFe] hydrogenase maturation protein
• UniProt Protein Name: Carbamoyltransferase HypF
• Protein Family: Carbamoyltransferase
• UniProt Gene Name: hypF
• UniProt Synonym Gene Names: Hydrogenase maturation protein HypF

NCBI Description

FhlA regulon. [More information is available at EcoGene: EG11551]. NiFe-hydrogenases have one CO and two CN groups bound as ligands to the iron. [More information is available at EcoCyc: EG11551].

Uniprot Description

Involved in the maturation of [NiFe] hydrogenases (PubMed:8661925, PubMed:12377778, PubMed:12586941). Along with HypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases (PubMed:12586941). HypF functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carboxamido moiety in an ATP-dependent reaction to the thiolate of the C-terminal cysteine of HypE yielding a protein-S-carboxamide (PubMed:12586941, PubMed:15291820). In the absence of any other substrate, displays carbamoyl-phosphate phosphatase activity (PubMed:12377778).