Rabbit HYAL1 Polyclonal Antibody | anti-HYAL1 antibody

HYAL1 Polyclonal Antibody

Cat. Num. AAA3011630

• Gene Names: HYAL1; MPS9; NAT6; LUCA1; HYAL-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: HYAL1; Polyclonal Antibody; HYAL1 Polyclonal Antibody; Hyaluronidase-1; Hyal-1; Hyaluronoglucosaminidase-1; Lung carcinoma protein 1; LuCa-1; LUCA1; anti-HYAL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: HYAL1 polyclonal antibody detects endogenous levels of HYAL1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 435

• Applicable Applications for anti-HYAL1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant full length Human HYAL1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver injury using HYAL1 antibody at dilution of 1:100 (x40 lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human gastric using HYAL1 antibody at dilution of 1:100 (x40 lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat kidney using HYAL1 antibody at dilution of 1:100 (x40 lens).)

Related Product Information for anti-HYAL1 antibody

Hyaluronidases (HAases or HYALs) are a family of lysosomal enzymes that are crucial for the spread of bacterial infections and of toxins present in a variety of venoms. HYALs may also be involved in the progression of cancer. In humans, six HYAL proteins have been identified. Most HYAL proteins degrade hyaluronic acid (HA), which is present in body fluids, tissues and the extracellular matrix of vertebrate tissues. HA keeps tissues hydrated, maintains osmotic balance and promotes cell proliferation, differentiation and metastasis. HA is also an important structural component of cartilage and other tissues and acts as a lubricant in joints. HYAL1 is a 435 amino acid hyaluronidase that is is expressed in multiple tissues, specifically in the serum, and is not expressed in brain. HYAL1 degrades HA into into fragments that stimulate angiogenesis. Expression of HYAL1 in various cancer cells may have a role in the regulation of tumor growth and progression.

NCBI and Uniprot Product Information

• NCBI GI #: 74735617
• NCBI GeneID: 3373
• UniProt Accession #: Q12794
• Molecular Weight: ~ 48kDa
• NCBI Official Full Name: Hyaluronidase-1
• NCBI Official Synonym Full Names: hyaluronidase 1
• NCBI Official Symbol: HYAL1
• NCBI Official Synonym Symbols: MPS9; NAT6; LUCA1; HYAL-1
• NCBI Protein Information: hyaluronidase-1
• UniProt Protein Name: Hyaluronidase-1
• UniProt Gene Name: HYAL1
• UniProt Synonym Gene Names: LUCA1; Hyal-1; LuCa-1
• UniProt Entry Name: HYAL1_HUMAN

NCBI Description

This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HYAL1: May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth. Defects in HYAL1 are the cause of mucopolysaccharidosis type 9 (MPS9); also called hyaluronidase deficiency. MPS9 is a lysosomal storage disease characterized by high hyaluronan (HA) concentration in the serum. The clinical features are periarticular soft tissue masses, mild short stature and acetabular erosions, and absence of neurological or visceral involvement. Belongs to the glycosyl hydrolase 56 family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.2.1.35; Glycan Metabolism - glycosaminoglycan degradation; Secreted, signal peptide; Secreted; Hydrolase

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: extracellular space; lysosomal lumen; lysosome; cytoplasm; cytoplasmic vesicle

Molecular Function: viral receptor activity; hyaluronan synthase activity; transcription factor binding; hyalurononglucosaminidase activity

Biological Process: positive regulation of cell adhesion; glycosaminoglycan metabolic process; response to virus; pathogenesis; positive regulation of cell growth; hyaluronan catabolic process; response to antibiotic; chondroitin sulfate metabolic process; positive regulation of angiogenesis; response to reactive oxygen species; hyaluronan biosynthetic process; cartilage development; carbohydrate metabolic process; chondroitin sulfate catabolic process; negative regulation of cell growth; inflammatory response; hyaluronan metabolic process; positive regulation of growth; positive regulation of epithelial cell proliferation

Disease: Mucopolysaccharidosis, Type Ix