Rabbit HUS1 Polyclonal Antibody | anti-HUS1 antibody

HUS1 Polyclonal Antibody

Cat. Num. AAA2555248

• Gene Names: HUS1; hHUS1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: HUS1; Polyclonal Antibody; HUS1 Polyclonal Antibody; Checkpoint protein HUS1; hHUS1; HUS1 (S. pombe) checkpoint homolog; Hus1; HUS1 checkpoint homolog (S. pombe); HUS1 Checkpoint Protein; HUS1 protein; HUS1+-like protein; Hydroxyurea-sensitive 1; S. pombe; homolog of; anti-HUS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 259

• Applicable Applications for anti-HUS1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human HUS1
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of mouse kidney, using HUS1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat liver using HUS1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human gastric cancer using HUS1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human uterine cancer using HUS1 antibody.)

Related Product Information for anti-HUS1 antibody

The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-HUS1 antibody

Cancer; Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 4758576
• NCBI GeneID: 3364
• NCBI Accession #: NP_004498.1
• NCBI GenBank Nucleotide #: NM_004507.4
• UniProt Accession #: O60921; B4DFI9
• Molecular Weight: Calculated: 29kDa; 31kDa; Observed: 32kDa
• NCBI Official Full Name: checkpoint protein HUS1 isoform 1
• NCBI Official Synonym Full Names: HUS1 checkpoint clamp component
• NCBI Official Symbol: HUS1
• NCBI Official Synonym Symbols: hHUS1
• NCBI Protein Information: checkpoint protein HUS1
• UniProt Protein Name: Checkpoint protein HUS1
• Protein Family: Checkpoint protein
• UniProt Gene Name: HUS1
• UniProt Synonym Gene Names: hHUS1

NCBI Description

The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011]

Uniprot Description

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase.