Western Blot (WB)
(Gel: 12%SDS-PAGE Lysate: 40 μ g Lane: K562 cell Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds)
Rabbit anti-Human HUS1 Polyclonal Antibody | anti-HUS1 antibody
HUS1 Antibody
Immunohistochemistry: 1:25-1:100
Western Blot (WB)
(Gel: 12%SDS-PAGE Lysate: 40 μ g Lane: K562 cell Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds)
Western Blot (WB)
(Gel: 12%SDS-PAGE Lysate: 40 μ g Lane: K562 cell Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds)
NCBI and Uniprot Product Information
Uniprot Description
HUS1: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Belongs to the HUS1 family.
Protein type: Cell cycle regulation; DNA repair, damage
Chromosomal Location of Human Ortholog: 7p13-p12
Cellular Component: nucleoplasm; nucleus
Molecular Function: protein binding
Biological Process: DNA damage checkpoint; DNA repair; DNA replication; double-strand break repair via homologous recombination; intra-S DNA damage checkpoint; mitotic cell cycle DNA replication checkpoint; nucleotide-excision repair; response to DNA damage stimulus
Similar Products
Product Notes
The HUS1 hus1 (Catalog #AAA9416382) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HUS1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HUS1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:200-1:1000 Immunohistochemistry: 1:25-1:100. Researchers should empirically determine the suitability of the HUS1 hus1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HUS1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
