Immunohistochemistry (IHC)
(Sample Data: Immunohistochemical analysis of formalin-fixed and paraffin embedded human brain cerebellum using Anti-Phospho-HTT (Ser421) (1:100 dilution).)
Rabbit anti-Human HTT Polyclonal Antibody | anti-phospho-HTT antibody
Anti-phospho-HTT (Ser421)
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Affinity chromatography
Synonyms
HTT; Polyclonal Antibody; Anti-phospho-HTT (Ser421); anti-phospho-HTT antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the human Huntington protein phosphorylated at Serine 421
Purity/Purification
Affinity chromatography
Form/Format
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH7.2 + 0.01% (w/v) Sodium Azide
Applicable Applications for anti-phospho-HTT antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Immunogen
The antibody was produced against synthesized peptide corresponding to amino acids 416-424 of human Huntington protein.
Preparation and Storage
1 year at -20 degree C from date of shipment
Immunohistochemistry (IHC)
(Sample Data: Immunohistochemical analysis of formalin-fixed and paraffin embedded human brain cerebellum using Anti-Phospho-HTT (Ser421) (1:100 dilution).)
Immunohistochemistry (IHC)
(Sample Data: Immunohistochemical analysis of formalin-fixed and paraffin embedded human brain cerebellum using Anti-Phospho-HTT (Ser421) (1:100 dilution).)
Western Blot (WB)
(Sample Data: Western blot analysis on endogenous protein present in unstimulated human PC-3 whole cell lysate using Anti-Phospho-HTT (Ser421) antibody (1:1,000 dilution).)
Western Blot (WB)
(Sample Data: Western blot analysis on endogenous protein present in unstimulated human PC-3 whole cell lysate using Anti-Phospho-HTT (Ser421) antibody (1:1,000 dilution).)
Related Product Information for anti-phospho-HTT antibody
Huntingtin (also known as Huntington's disease protein, Htt and HD protein) is the protein product of a disease gene linked to Huntington's disease, a neuro-degenerative disorder characterized by loss of striatal neurons. This may be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product (see partial protein sequence below). The huntingtin gene locus is large, spanning 180 kb and consisting of 67 exons. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. Normal huntingtin protein shows a cytoplasmic localization. This protein is widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.
Product Categories/Family for anti-phospho-HTT antibody
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Product Notes
The phospho-HTT (Catalog #AAA515995) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-phospho-HTT (Ser421) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HTT can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). Researchers should empirically determine the suitability of the phospho-HTT for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HTT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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